Protein kinetics via UV/VIS and FTIR imaging on chip

M. W. Toepke, S. H. Brewer, D. M. Vu, J. E. Morgan, K. Ganesan, K. D. Rector, W. H. Woodruff, R. B. Gennis, R. B. Dyer, P. J.A. Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microfluidic flow cells for kinetic studies of proteins using spectroscopic imaging are presented. Reactions were initiated using an argon ion laser and time-resolved kinetics were obtained by observing the spectrum at several points down the length of the microfluidic channel in the direction of fluid flow. Dispersion within samples was minimized using a sheath flow of buffer around the analyte solution.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages1464-1466
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - Jan 1 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Fingerprint

Microfluidics
Proteins
Imaging techniques
Kinetics
Argon
Flow of fluids
Buffers
Ions
Lasers
Direction compound

Keywords

  • Kinetics
  • Microfluidics
  • Spectroscopy
  • Time-resolved

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Toepke, M. W., Brewer, S. H., Vu, D. M., Morgan, J. E., Ganesan, K., Rector, K. D., ... Kenis, P. J. A. (2005). Protein kinetics via UV/VIS and FTIR imaging on chip. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1464-1466). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.

Protein kinetics via UV/VIS and FTIR imaging on chip. / Toepke, M. W.; Brewer, S. H.; Vu, D. M.; Morgan, J. E.; Ganesan, K.; Rector, K. D.; Woodruff, W. H.; Gennis, R. B.; Dyer, R. B.; Kenis, P. J.A.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. p. 1464-1466 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Toepke, MW, Brewer, SH, Vu, DM, Morgan, JE, Ganesan, K, Rector, KD, Woodruff, WH, Gennis, RB, Dyer, RB & Kenis, PJA 2005, Protein kinetics via UV/VIS and FTIR imaging on chip. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, vol. 1, Transducer Research Foundation, pp. 1464-1466, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 10/9/05.
Toepke MW, Brewer SH, Vu DM, Morgan JE, Ganesan K, Rector KD et al. Protein kinetics via UV/VIS and FTIR imaging on chip. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation. 2005. p. 1464-1466. (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Toepke, M. W. ; Brewer, S. H. ; Vu, D. M. ; Morgan, J. E. ; Ganesan, K. ; Rector, K. D. ; Woodruff, W. H. ; Gennis, R. B. ; Dyer, R. B. ; Kenis, P. J.A. / Protein kinetics via UV/VIS and FTIR imaging on chip. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. pp. 1464-1466 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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