Protein kinetics via spectroscopic imaging on a microfluidic chip

Michael W. Toepke, Scott H. Brewer, Joel E. Morgan, Dung M. Vu, Krithika Ganesan, Kirk D. Rector, William H. Woodruff, Robert B Gennis, R. Brian Dyer, Paul J A Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microfluidic devices can be invaluable tools to minimize the amount of analyte or sample needed for a certain experiment. Sample conservation is of special interest when analyzing precious materials, such as proteins that can only be optioned in vary small quantities after extensive purification efforts. The present work demonstrates the use of microfluidic devices for time-resolved spectroscopic studies. Time-resolved spectroscopy can be achieved in microfluidic devices by activating a sample in a channel and using the length down the channel as time resolution. This talk will cover the design, fabrication and operation of microfluidic chips that enable rapid mixing of samples while reducing sample dispersion due to parabolic flow profiles. In addition, the fabrication of a custom calcium fluoride flow cell, transparent in the IR, will be presented. The results of time-resolved UV/Vis and FTIR studies of myoglobin and cytochrome oxidase obtained with these microfluidic chips will be discussed.

Original languageEnglish (US)
Title of host publication05AIChE
Subtitle of host publication2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings
Pages12694
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Toepke, M. W., Brewer, S. H., Morgan, J. E., Vu, D. M., Ganesan, K., Rector, K. D., Woodruff, W. H., Gennis, R. B., Dyer, R. B., & Kenis, P. J. A. (2005). Protein kinetics via spectroscopic imaging on a microfluidic chip. In 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings (pp. 12694)