Protein kinase C phosphorylates leukemia RNA polymerase II

Linda F. Chuang, Ronald H. Cooper, Peter M Yau, E. Morton Bradbury, Ronald Y. Chuang

Research output: Contribution to journalArticle

Abstract

Purified RNA polymerase II from chicken leukemia cells was found to be an effective substrate for protein kinase C but not cAMP-dependent protein kinase. Protein kinase C catalyzed the incorporation of 1-2 mol of phosphate per mol of polymerase II and the reaction was totally calcium and lipid dependent. Electrophoresis studies revealed a time-dependent increase of phosphate incorporation into RNA polymerase II subunits of 220 KDa, 180 KDa and 150 KDa, with a preferential phosphorylation of the 180 KDa polypeptide. The phosphorylated enzyme has a preference for using single-stranded DNA as the template for transcription, including transcription of the single-stranded myb oncogene sequence. Phosphoamino acid analysis indicated that both serine and threonine residues were phosphorylated at equal amounts. Phosphorylation by protein kinase C increased the affinity of substrate-polymerase binding and the initial rate of RNA synthesis, suggesting a mechanism by which gene expression can be activated by protein kinase C.

Original languageEnglish (US)
Pages (from-to)1376-1383
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume145
Issue number3
DOIs
StatePublished - Jun 30 1987
Externally publishedYes

Fingerprint

RNA Polymerase II
Protein Kinase C
Leukemia
Phosphorylation
Transcription
myb Genes
Phosphates
Phosphoamino Acids
Single-Stranded DNA
Substrates
Threonine
Cyclic AMP-Dependent Protein Kinases
Electrophoresis
Gene expression
Serine
Chickens
RNA
Calcium
Lipids
Gene Expression

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Protein kinase C phosphorylates leukemia RNA polymerase II. / Chuang, Linda F.; Cooper, Ronald H.; Yau, Peter M; Bradbury, E. Morton; Chuang, Ronald Y.

In: Biochemical and Biophysical Research Communications, Vol. 145, No. 3, 30.06.1987, p. 1376-1383.

Research output: Contribution to journalArticle

Chuang, Linda F. ; Cooper, Ronald H. ; Yau, Peter M ; Bradbury, E. Morton ; Chuang, Ronald Y. / Protein kinase C phosphorylates leukemia RNA polymerase II. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 145, No. 3. pp. 1376-1383.
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