Abstract
Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed "protein induced fluorescence enhancement" (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.
Original language | English (US) |
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Pages (from-to) | 1221-1229 |
Number of pages | 9 |
Journal | Chemical Society Reviews |
Volume | 43 |
Issue number | 4 |
DOIs | |
State | Published - Feb 21 2014 |
ASJC Scopus subject areas
- General Chemistry