Protein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions

Helen Hwang, Su-A Myong

Research output: Contribution to journalReview article

Abstract

Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed "protein induced fluorescence enhancement" (PIFE). Unlike FRET where two dyes are required, PIFE employs a single dye attached to DNA or RNA to which an unlabeled protein is applied. We discuss both ensemble and single molecule studies in which PIFE was utilized.

Original languageEnglish (US)
Pages (from-to)1221-1229
Number of pages9
JournalChemical Society Reviews
Volume43
Issue number4
DOIs
StatePublished - Feb 21 2014

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Nucleic Acids
Fluorescence
Proteins
Molecules
Coloring Agents
RNA
DNA
Kinetics
Substrates

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Protein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions. / Hwang, Helen; Myong, Su-A.

In: Chemical Society Reviews, Vol. 43, No. 4, 21.02.2014, p. 1221-1229.

Research output: Contribution to journalReview article

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