Protein hydrolysates from β-Conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro

Cristina Martinez-Villaluenga, Vermont P. Dia, Mark Berhow, Neal A. Bringe, Elvira Gonzalez de Mejia

Research output: Contribution to journalArticle

Abstract

Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory effect of SPH produced by alcalase on LPS-induced macrophage RAW 264.7 cell line was also investigated. SAH (100 lM) derived from soybean enriched in β-conglycinin (BC) (up to 47% total pro-tein) decreased lipid accumulation (33-37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 lM) inhibited lipid accumulation to a lesser extent (8-14% inhibition) through inhibition of LPL gene expression. SAH (5 lM) decreased the production of nitric oxide (NO) (18-35%) and prostaglandin E2 (PGE2) (47-71%) and the expression of inducible nitric oxide synthase (iNOS) (31-53%) and cycloxygenase-2 (COX-2) (30-52%). This is the first investigation showing that soy hydrolysates inhibit LPS-induced iNOS/ NO and COX-2/PGE2 pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.

Original languageEnglish (US)
Pages (from-to)1007-1018
Number of pages12
JournalMolecular Nutrition and Food Research
Volume53
Issue number8
DOIs
StatePublished - Aug 1 2009

Keywords

  • Biomarkers
  • Inflammation
  • Lipid metabolism
  • Soy protein hydrolysates
  • β-conglycinin

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Fingerprint Dive into the research topics of 'Protein hydrolysates from β-Conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro'. Together they form a unique fingerprint.

  • Cite this