Protein folding landscapes in the living cell

Simon Ebbinghaus, Martin Gruebele

Research output: Contribution to journalReview article

Abstract

This Perspective highlights studies of protein structure, stability, and folding kinetics that can now be carried out in prokaryotic and eukaryotic cells. The general features of cooperative folding transitions and kinetics remain the same as those in vitro, but the folding free-energy landscape and local viscosity vary according to microenvironments in the cellular landscape. Experiments in vitro provide a basis for comparing crowding and chemical effects with experiments inside of the cell. Perhaps cells even evolved to post-translationally modulate protein folding and binding through chemical patterns or by crowding.

Original languageEnglish (US)
Pages (from-to)314-319
Number of pages6
JournalJournal of Physical Chemistry Letters
Volume2
Issue number4
DOIs
StatePublished - Feb 17 2011

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Protein folding
folding
Cells
proteins
Kinetics
crowding
Free energy
cells
Experiments
Viscosity
Proteins
chemical effects
kinetics
free energy
viscosity
Protein Binding

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Protein folding landscapes in the living cell. / Ebbinghaus, Simon; Gruebele, Martin.

In: Journal of Physical Chemistry Letters, Vol. 2, No. 4, 17.02.2011, p. 314-319.

Research output: Contribution to journalReview article

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