Protein binding kinetics quantification via coupled plasmonic-photonic resonance nanosensors in generic microplate reader

Tang Dang, Wenjun Hu, Wei Zhang, Zifang Song, Yi Wang, Mingqian Chen, Hao Xu, Gang Logan Liu

Research output: Contribution to journalArticle

Abstract

Almost no analytical assays, either colorimetric or fluorescence assays, for generic microplate readers is capable of dynamic measurements of protein-protein binding or the quantification of kinetic association and dissociation constants of protein interactions. On the other hand, protein binding kinetics quantification can be uniquely done on special expensive surface plasmon resonance (SPR) sensing equipment. Here we report the integration of coupled plasmonic-photonic resonance nanosensors in standard 96-well plate format and by using which, for the very first time, the demonstration of label-free dynamic SPR-like protein binding measurement and kinetics quantification in a generic microplate reader. Our low-cost label-free nanosensor plate enables very sensitive detection of immobilized protein interactions based on the transmission optical density (OD) value changes at specific wavelengths measured in a generic microplate reader. The relative end-point OD value changes show a good linear response with protein concentrations (from 0.05 to 50 μg/ml). And the protein quantification in serum results are consistent with the concurrent hospital lab tests. Most importantly, the kinetic association and dissociation constants of protein interactions in our sensor plate wells are determined by time-lapse dynamic OD value measurement in the generic microplate reader. Enabled by our unique nanosensor plate, SPR-like measurement of protein binding kinetics is now available using generic microplate reader ubiquitous in many chemistry and biomedical research labs.

Original languageEnglish (US)
Article number111494
JournalBiosensors and Bioelectronics
Volume142
DOIs
StatePublished - Oct 1 2019

Fingerprint

Optics and Photonics
Nanosensors
Protein Binding
Photonics
Density (optical)
Surface Plasmon Resonance
Proteins
Surface plasmon resonance
Kinetics
Labels
Assays
Association reactions
Immobilized Proteins
Biomedical Research
Demonstrations
Fluorescence
Costs and Cost Analysis
Equipment and Supplies
Wavelength
Sensors

Keywords

  • C-reactive protein
  • Nanophotonics
  • Protein binding kinetics
  • Sandwich immunoassay
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Protein binding kinetics quantification via coupled plasmonic-photonic resonance nanosensors in generic microplate reader. / Dang, Tang; Hu, Wenjun; Zhang, Wei; Song, Zifang; Wang, Yi; Chen, Mingqian; Xu, Hao; Liu, Gang Logan.

In: Biosensors and Bioelectronics, Vol. 142, 111494, 01.10.2019.

Research output: Contribution to journalArticle

Dang, Tang ; Hu, Wenjun ; Zhang, Wei ; Song, Zifang ; Wang, Yi ; Chen, Mingqian ; Xu, Hao ; Liu, Gang Logan. / Protein binding kinetics quantification via coupled plasmonic-photonic resonance nanosensors in generic microplate reader. In: Biosensors and Bioelectronics. 2019 ; Vol. 142.
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