Protein adsorption properties of OEG monolayers and dense PNIPAM brushes probed by neutron reflectivity

N. Brouette, C. Xue, M. Haertlein, M. Moulin, G. Fragneto, D. E. Leckband, A. Halperin, M. Sferrazza

Research output: Contribution to journalArticle

Abstract

The structure of dense poly(N-isopropylacrylamide) (PNIPAM) brushes and oligo(ethylene glycol) (OEG) monolayers has been probed using neutron reflectometry and ellipsometry. The PNIPAM brush is swollen below the Lower Critical Solution Temperature (LCST) of 32 {ring operator}C and is collapsed at 37 {ring operator}C. Neutron reflectivity shows that below the LCST, the brush is described by a two-layer model: an inner dense layer and a hydrated outer layer. Above the LCST the collapsed brush forms a homogenous layer. With a fully deuterated myoglobin protein to increase the neutron scattering length density contrast, the reflectivity data show no detectable primary adsorption on the grafted OEG surface. A bound on the ternary adsorption onto PNIPAM chains forming dense brushes below and above the LCST is obtained.

Original languageEnglish (US)
Pages (from-to)343-353
Number of pages11
JournalEuropean Physical Journal: Special Topics
Volume213
Issue number1
DOIs
StatePublished - Dec 1 2012

ASJC Scopus subject areas

  • Materials Science(all)
  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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