Protective action of Ilex paraguariensis extract against free radical inactivation of paraoxonase-1 in high-density lipoprotein

Teresita Menini, Caleb Heck, John Schulze, Elvira Demejia, Alejandro Gugliucci

Research output: Contribution to journalArticle

Abstract

Paraoxonase 1 (PON-1), an antioxidant enzyme carried mainly by HDL, has been shown to display cardioprotective effects. In this study, we investigated whether the polyphenol-rich beverage mate, obtained from extract of Ilex paraguariensis (IP), prevented the loss of PON-1 activity from HDL during oxidant stress. The peroxide radical generator 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH) induced time- and dose-dependent oxidation of HDL, as measured by lipoperoxide content, which is accompanied by a parallel decrease in the activity of PON-1 (p < 0.001). IP extract (2-20 μL/mL) afforded time- and concentration-dependent inhibition of the oxidation, with preservation of apoA-I structure and PON-1 activity. Healthy volunteers drank either 0.5 L of IP extract, 0.5 L of coffee and milk or nothing. PON-1 activity increased an average of 10% above the changes seen when the intake was coffee and milk (p < 0.05). In conclusion, we demonstrate that IP extract may, to some extent, prevent the loss of the antiatherogenic function of HDL afforded by PON-1 when the particle is under oxidant stress.

Original languageEnglish (US)
Pages (from-to)1141-1147
Number of pages7
JournalPlanta Medica
Volume73
Issue number11
DOIs
StatePublished - Sep 1 2007

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Ilex paraguariensis
Aryldialkylphosphatase
HDL Lipoproteins
Free Radicals
Coffee
Oxidants
Milk
Oxidation
Beverages
Lipid Peroxides
Apolipoprotein A-I
Peroxides
Polyphenols
Healthy Volunteers
Antioxidants
Enzymes

Keywords

  • Aquifoliaceae
  • Atherosclerosis
  • HDL
  • Ilex paraguariensis
  • Oxidative stress
  • Paraoxonase 1
  • Polyphenol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Protective action of Ilex paraguariensis extract against free radical inactivation of paraoxonase-1 in high-density lipoprotein. / Menini, Teresita; Heck, Caleb; Schulze, John; Demejia, Elvira; Gugliucci, Alejandro.

In: Planta Medica, Vol. 73, No. 11, 01.09.2007, p. 1141-1147.

Research output: Contribution to journalArticle

Menini, Teresita ; Heck, Caleb ; Schulze, John ; Demejia, Elvira ; Gugliucci, Alejandro. / Protective action of Ilex paraguariensis extract against free radical inactivation of paraoxonase-1 in high-density lipoprotein. In: Planta Medica. 2007 ; Vol. 73, No. 11. pp. 1141-1147.
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