Proofreading of noncognate Acyl adenylates by an acyl-coenzyme a ligase

Miglena Manandhar, John E. Cronan

Research output: Contribution to journalArticlepeer-review


Summary Aminoacyl-tRNA synthetases remove (proofread) incorrect substrates and thereby prevent errors in protein synthesis. We report enzyme-catalyzed pretransfer editing by pimeloyl-coenzyme A (CoA) ligase (BioW), a biotin synthetic enzyme that converts pimelate, a seven-carbon dicarboxylic acid, to its CoA ester. The noncognate BioW substrate glutaric acid results in hydrolysis of ATP to AMP with formation of only trace amounts of glutaryl-CoA, thereby mimicking pretransfer editing of incorrect aminoacyl-adenylates by aminoacyl-tRNA synthetases.

Original languageEnglish (US)
Pages (from-to)1441-1446
Number of pages6
JournalChemistry and Biology
Issue number12
StatePublished - Dec 19 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry


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