Proliferative dermatitis in a loggerhead turtle, Caretta caretta, and a green turtle, Chelonia mydas, associated with novel papillomaviruses

Charles A. Manire, Brian A. Stacy, Michael J Kinsel, Heather T. Daniel, Eric T. Anderson, James F.X. Wellehan

Research output: Contribution to journalArticlepeer-review

Abstract

A subadult loggerhead turtle, Caretta caretta, presented with generalized small, white, raised lesions over its neck, shoulders, and all four flippers. A juvenile green turtle, Chelonia mydas, recently treated for fibropapillomatosis, presented with four similar localized lesions on one flipper. To diagnose the conditions, biopsies of the lesions were taken for histopathology, electron microscopy, and molecular diagnostics. Histopathologic findings were similar in the two turtles and skin lesions were characterized by multifocal areas of epidermal hyperplasia accompanied by variation and abnormalities in the nuclear morphology of keratinocytes and a few intranuclear inclusions in some cells. Transmission electron microscopy revealed multiple epithelial cells with large intranuclear aggregates of virions consistent in morphology with papillomavirus. Papillomavirus was detected in samples from both turtles by polymerase chain reaction (PCR). Sequence analysis of the partial sequence of the papillomavirus E1 gene revealed two viruses (CcPV and CmPV) that were distinct from each other and from other species in Papillomaviridae, and likely represent two novel species and perhaps a new genus.

Original languageEnglish (US)
Pages (from-to)227-237
Number of pages11
JournalVeterinary Microbiology
Volume130
Issue number3-4
DOIs
StatePublished - Aug 25 2008

Keywords

  • Caretta caretta
  • Chelonia mydas
  • Green turtle
  • Loggerhead turtle
  • PCR
  • Papillomavirus
  • Reptile

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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