Proinflammatory roles of T-cell receptor (TCR)γδ and TCRαβ lymphocytes in a murine model of asthma

Craig M. Schramm, Lynn Puddington, Carmen A. Yiamouyiannis, Elizabeth G. Lingenheld, Herbert E. Whiteley, Walter W. Wolyniec, Thomas C. Noonan, Roger S. Thrall

Research output: Contribution to journalArticlepeer-review


The role of lymphocytes bearing αβ or γδ T-cell receptors (TCRs) was assessed during the acute allergic response in a mouse model of asthma. The inflammatory immune response to ovalbumin (OVA) was characterized in wild-type C57BL/6J mice and congenic TCRβ(-/-) and TCRδ(-/-) mice by evaluation of airway eosinophilia, histopathology, serum immunoglobulin (Ig)E levels, and in vivo airway responsiveness to methacholine. OVA-challenged wild-type mice demonstrated marked pulmonary inflammation, evidenced by airway eosinophilia (68 ± 7 X 104 cells), peribronchial lympho-plasmocytic infiltration, and elevated serum IgE (4.9 ± 0.6 μg/ml). These responses were markedly attenuated in TCRδ(-/-) animals (5.0 ± 1.0 X 104 eosinophils and 1.6 ± 0.3 μg/ml IgE) and were completely absent in TCRβ(-/-) mice (< 1 x 103 eosinophils and 0.38 ± 0.21 μg/ml IgE). Similar results were observed in mice treated with anti-TCRγδ or anti-TCRαβ monoclonal antibodies. Airway responsiveness to aerosolized methacholine was also reduced in challenged TCRδ(-/-) animals relative to challenged wild-type mice. These results demonstrate that acute allergic airway responses are dependent upon intact TCRαβ and TCRγδ lymphocyte function and that TCRγδ cells promote acute airway sensitization.

Original languageEnglish (US)
Pages (from-to)218-225
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Issue number2
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology


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