Abstract
The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 95-104 |
| Number of pages | 10 |
| Journal | Current Opinion in Biotechnology |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2013 |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
Progress toward single cell metabolomics. / Rubakhin, Stanislav; Lanni, Eric J.; Sweedler, Jonathan V.
In: Current Opinion in Biotechnology, Vol. 24, No. 1, 01.02.2013, p. 95-104.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Progress toward single cell metabolomics
AU - Rubakhin, Stanislav
AU - Lanni, Eric J.
AU - Sweedler, Jonathan V
PY - 2013/2/1
Y1 - 2013/2/1
N2 - The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.
AB - The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.
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UR - http://www.scopus.com/inward/citedby.url?scp=84872270965&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2012.10.021
DO - 10.1016/j.copbio.2012.10.021
M3 - Review article
C2 - 23246232
AN - SCOPUS:84872270965
VL - 24
SP - 95
EP - 104
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
SN - 0958-1669
IS - 1
ER -