Abstract

The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.

Original languageEnglish (US)
Pages (from-to)95-104
Number of pages10
JournalCurrent Opinion in Biotechnology
Volume24
Issue number1
DOIs
StatePublished - Feb 1 2013

Fingerprint

Metabolomics
Metabolites
Molecules
Microfluidics
Mass spectrometry
Cells
Throughput
Metabolome
Mass Spectrometry
Sensitivity and Specificity
Population

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Progress toward single cell metabolomics. / Rubakhin, Stanislav; Lanni, Eric J.; Sweedler, Jonathan V.

In: Current Opinion in Biotechnology, Vol. 24, No. 1, 01.02.2013, p. 95-104.

Research output: Contribution to journalReview article

@article{c914a9df7d944ad49909f489afb30a24,
title = "Progress toward single cell metabolomics",
abstract = "The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.",
author = "Stanislav Rubakhin and Lanni, {Eric J.} and Sweedler, {Jonathan V}",
year = "2013",
month = "2",
day = "1",
doi = "10.1016/j.copbio.2012.10.021",
language = "English (US)",
volume = "24",
pages = "95--104",
journal = "Current Opinion in Biotechnology",
issn = "0958-1669",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Progress toward single cell metabolomics

AU - Rubakhin, Stanislav

AU - Lanni, Eric J.

AU - Sweedler, Jonathan V

PY - 2013/2/1

Y1 - 2013/2/1

N2 - The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.

AB - The metabolome refers to the entire set of small molecules, or metabolites, within a biological sample. These molecules are involved in many fundamental intracellular functions and reflect the cell's physiological condition. The ability to detect and identify metabolites and determine and monitor their amounts at the single cell level enables an exciting range of studies of biological variation and functional heterogeneity between cells, even within a presumably homogenous cell population. Significant progress has been made in the development and application of bioanalytical tools for single cell metabolomics based on mass spectrometry, microfluidics, and capillary separations. Remarkable improvements in the sensitivity, specificity, and throughput of these approaches enable investigation of multiple metabolites simultaneously in a range of individual cell samples.

UR - http://www.scopus.com/inward/record.url?scp=84872270965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872270965&partnerID=8YFLogxK

U2 - 10.1016/j.copbio.2012.10.021

DO - 10.1016/j.copbio.2012.10.021

M3 - Review article

C2 - 23246232

AN - SCOPUS:84872270965

VL - 24

SP - 95

EP - 104

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

SN - 0958-1669

IS - 1

ER -