Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880

Sujit Sadashiv Jagtap, Ashwini Ashok Bedekar, Jing Jing Liu, Yong Su Jin, Christopher V. Rao

Research output: Contribution to journalArticlepeer-review


Background: Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols. Results: Rhodosporidium toruloides is able to produce galactitol from galactose. During growth in nitrogen-rich medium, R. toruloides produced 3.2 ± 0.6 g/L, and 8.4 ± 0.8 g/L galactitol from 20 to 40 g/L galactose, respectively. In addition, R. toruloides was able to produce galactitol from galactose at reduced titers during growth in nitrogen-poor medium, which also induces lipid production. These results suggest that R. toruloides can potentially be used for the co-production of lipids and galactitol from galactose. We further characterized the mechanism for galactitol production, including identifying and biochemically characterizing the critical aldose reductase. Intracellular metabolite analysis was also performed to further understand galactose metabolism. Conclusions: Rhodosporidium toruloides has traditionally been used for the production of lipids and lipid-based chemicals. Our work demonstrates that R. toruloides can also produce galactitol, which can be used to produce polymers with applications in medicine and as a precursor for anti-cancer drugs. Collectively, our results further establish that R. toruloides can produce multiple value-added chemicals from a wide range of sugars.

Original languageEnglish (US)
Article number250
JournalBiotechnology for Biofuels
Issue number1
StatePublished - Oct 18 2019


  • Aldose reductase
  • Galactitol
  • Galactose
  • Lipid
  • Metabolite profiling
  • Rhodosporidium toruloides

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Energy(all)
  • Management, Monitoring, Policy and Law


Dive into the research topics of 'Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880'. Together they form a unique fingerprint.

Cite this