Production of Designer Xylose-Acetic Acid Enriched Hydrolysate from Bioenergy Sorghum, Oilcane, and Energycane Bagasses

Ming Hsun Cheng, Shuchi Singh, Aidan N. Carr Clennon, Bruce S. Dien, Vijay Singh

Research output: Contribution to journalArticlepeer-review

Abstract

Xylan accounts for up to 40% of the structural carbohydrates in lignocellulosic feedstocks. Along with xylan, acetic acid in sources of hemicellulose can be recovered and marketed as a commodity chemical. Through vibrant bioprocessing innovations, converting xylose and acetic acid into high-value bioproducts via microbial cultures improves the feasibility of lignocellulosic biorefineries. Enzymatic hydrolysis using xylanase supplemented with acetylxylan esterase (AXE) was applied to prepare xylose-acetic acid enriched hydrolysates from bioenergy sorghum, oilcane, or energycane using sequential hydrothermal-mechanical pretreatment. Various biomass solids contents (15 to 25%, w/v) and xylanase loadings (140 to 280 FXU/g biomass) were tested to maximize xylose and acetic acid titers. The xylose and acetic acid yields were significantly improved by supplementing with AXE. The optimal yields of xylose and acetic acid were 92.29% and 62.26% obtained from hydrolyzing energycane and oilcane at 25% and 15% w/v biomass solids using 280 FXU xylanase/g biomass and AXE, respectively.

Original languageEnglish (US)
Article number129104
JournalBioresource Technology
Volume380
DOIs
StatePublished - Jul 2023

Keywords

  • Acetic acid
  • Acetylxylan esterase
  • Sequential hydrothermal-mechanical pretreatment
  • Xylanase
  • Xylose

ASJC Scopus subject areas

  • Bioengineering
  • Waste Management and Disposal
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment

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