Abstract
Fucose is a rare sugar obtained from fucoidan in brown algae. However, fucoidan contains low amounts of fucose, making the process of obtaining fucose inefficient and expensive. Colanic acid (CA) is a capsular exopolysaccharide produced by Escherichia coli, which has great potential as a source of fucose owing to its high proportion of fucose. In this study, we aimed to efficiently obtain fucose from the hydrolysis of CA and then produce 2′-fucosyllactose (2′-FL) and 2′-fucosylgalactose (2′-FG) using fucose in the CA hydrolysate, using engineered yeast. For the acid hydrolysis of CA, optimal conditions were 110 °C, 4% (w/v) H3PO4, and 60 min. Using fucose from CA hydrolysate as a substrate, 2′-FL and 2′-FG were produced from engineered Saccharomyces cerevisiae. This is the first report of producing fucosylated oligosaccharides from CA. Considering the benefits of CA in obtaining fucose, these results can lead to further applications of CA as a fucose supplier.
Original language | English (US) |
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Pages (from-to) | 332-340 |
Number of pages | 9 |
Journal | Process Biochemistry |
Volume | 122 |
DOIs | |
State | Published - Nov 2022 |
Keywords
- 2′-Fucosylgalactose
- 2′-Fucosyllactose
- Acid hydrolysis
- Colanic acid
- Fucose
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology