TY - JOUR
T1 - Production of an antigenic C4(V3)6 multiepitopic HIV protein in bacterial and plant systems
AU - Govea-Alonso, Dania O.
AU - Gómez-Cardona, Erik E.
AU - Rubio-Infante, Néstor
AU - García-Hernández, Ana Lilia
AU - Varona-Santos, Javier T.
AU - Salgado-Bustamante, Mariana
AU - Korban, Schuyler S.
AU - Moreno-Fierros, Leticia
AU - Rosales-Mendoza, Sergio
PY - 2013/4
Y1 - 2013/4
N2 - A C4(V3)6 multiepitopic protein was designed in an effort to pursue broad immunization against the human immunodeficiency virus (HIV). This C4(V3)6 chimeric protein is based on sequences of gp120, including epitopes from the fourth conserved domain (C4) and six tandem repeats of the third variable domain (V3), which represent different HIV isolates. The histidine-tagged C4(V3)6 was subsequently over-expressed in a recombinant Escherichia coli strain, and purified by immobilized metal ion affinity chromatography. Expression of the C4(V3)6 in both tobacco and lettuce plants was also achieved with no toxic effects on plant growth as transgenic plants were phenotypically normal. Moreover, the functional C4(V3)6 protein showed HIV antigenic determinants. The implications of these findings on the development of a new low-cost HIV vaccine are discussed.
AB - A C4(V3)6 multiepitopic protein was designed in an effort to pursue broad immunization against the human immunodeficiency virus (HIV). This C4(V3)6 chimeric protein is based on sequences of gp120, including epitopes from the fourth conserved domain (C4) and six tandem repeats of the third variable domain (V3), which represent different HIV isolates. The histidine-tagged C4(V3)6 was subsequently over-expressed in a recombinant Escherichia coli strain, and purified by immobilized metal ion affinity chromatography. Expression of the C4(V3)6 in both tobacco and lettuce plants was also achieved with no toxic effects on plant growth as transgenic plants were phenotypically normal. Moreover, the functional C4(V3)6 protein showed HIV antigenic determinants. The implications of these findings on the development of a new low-cost HIV vaccine are discussed.
KW - HIV vaccine
KW - Multiepitopic protein
KW - Plant-based vaccine
UR - http://www.scopus.com/inward/record.url?scp=84875437018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875437018&partnerID=8YFLogxK
U2 - 10.1007/s11240-012-0252-4
DO - 10.1007/s11240-012-0252-4
M3 - Article
AN - SCOPUS:84875437018
VL - 113
SP - 73
EP - 79
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -