Production of an antigenic C4(V3)6 multiepitopic HIV protein in bacterial and plant systems

Dania O. Govea-Alonso, Erik E. Gómez-Cardona, Néstor Rubio-Infante, Ana Lilia García-Hernández, Javier T. Varona-Santos, Mariana Salgado-Bustamante, Schuyler S. Korban, Leticia Moreno-Fierros, Sergio Rosales-Mendoza

Research output: Contribution to journalArticlepeer-review

Abstract

A C4(V3)6 multiepitopic protein was designed in an effort to pursue broad immunization against the human immunodeficiency virus (HIV). This C4(V3)6 chimeric protein is based on sequences of gp120, including epitopes from the fourth conserved domain (C4) and six tandem repeats of the third variable domain (V3), which represent different HIV isolates. The histidine-tagged C4(V3)6 was subsequently over-expressed in a recombinant Escherichia coli strain, and purified by immobilized metal ion affinity chromatography. Expression of the C4(V3)6 in both tobacco and lettuce plants was also achieved with no toxic effects on plant growth as transgenic plants were phenotypically normal. Moreover, the functional C4(V3)6 protein showed HIV antigenic determinants. The implications of these findings on the development of a new low-cost HIV vaccine are discussed.

Original languageEnglish (US)
Pages (from-to)73-79
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume113
Issue number1
DOIs
StatePublished - Apr 2013

Keywords

  • HIV vaccine
  • Multiepitopic protein
  • Plant-based vaccine

ASJC Scopus subject areas

  • Horticulture

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