Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping

Thaddeus C. Ezeji, Nasib Qureshi, Hans P. Blaschek

Research output: Contribution to journalArticlepeer-review

Abstract

A potential industrial substrate (liquefied corn starch; LCS) has been employed for successful acetone butanol ethanol (ABE) production. Fermentation of LCS (60 g l-1) in a batch process resulted in the production of 18.4 g l-1 ABE, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l-1 ABE). A batch fermentation of LCS integrated with product recovery resulted in 92% utilization of sugars present in the feed. When ABE was recovered by gas stripping (to relieve inhibition) from the fed-batch reactor fed with saccharified liquefied cornstarch (SLCS), 81.3 g l-1 ABE was produced compared to 18.6 g l-1 (control). In this integrated system, 225.8 g l-1 SLCS sugar (487 % of control) was consumed. In the absence of product removal, it is not possible for C. beijerinckii BA101 to utilize more than 46 g l-1 glucose. A combination of fermentation of this novel substrate (LCS) to butanol together with product recovery by gas stripping may economically benefit this fermentation.

Original languageEnglish (US)
Pages (from-to)771-777
Number of pages7
JournalJournal of Industrial Microbiology and Biotechnology
Volume34
Issue number12
DOIs
StatePublished - Dec 1 2007

Keywords

  • ABE fermentation
  • Clostridium beijerinckii BA101
  • Gas stripping
  • Liquefied cornstarch (LCS)
  • Saccharified liquefied cornstarch (SLCS)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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