Production of a hybridoma T suppressor cell and suppressor factor

J. E. Niederhuber, L. B. Schook, E. L. Bingham

Research output: Contribution to journalArticlepeer-review

Abstract

Two hybridoma T-cell lines were prepared by fusion of burro erythrocyte (BRBC)-specific T suppressor cells with the BW 5147 thymoma line. Culture supernatants from these T cell hybrids specifically suppressed the in vitro IgG antibody response to BRBC. Clones P3E8 and P4B10 were both removed via affinity columns bearing antibodies to BRBC and antibodies to I-J(k) subregion determinants. These results indicated that the hybridoma factors were I-J(k) determinant bearing and anti-idiotype determinant bearing. P3E8 supernatant was major histocompatibility complex (MHC) restricted, suppressing only H-2(k) haplotype spleen cells. Clone P4B10 supernatant was not MHC restricted, suppressing k, s, b, and d haplotype spleen cells. The properties of these two hybridoma factors would indicate they are derived from T(s)2 cells.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalSurgery
Volume92
Issue number2
StatePublished - Dec 1 1982
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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