Probing the molecular determinants of fluorinase specificity

W. L. Yeo, X. Chew, D. J. Smith, K. P. Chan, H. Sun, H. Zhao, Y. H. Lim, E. L. Ang

Research output: Contribution to journalArticle

Abstract

Molecular determinants of FlA1 fluorinase specificity were probed using 5′-chloro-5′-deoxyadenosine (5′-ClDA) analogs as substrates and FlA1 active site mutants. Modifications at F213 or A279 residues are beneficial towards these modified substrates, including 5′-chloro-5′-deoxy-2-ethynyladenosine, ClDEA (>10-fold activity improvement), and conferred novel activity towards substrates not readily accepted by wild-type FlA1.

Original languageEnglish (US)
Pages (from-to)2559-2562
Number of pages4
JournalChemical Communications
Volume53
Issue number17
DOIs
StatePublished - Jan 1 2017

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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  • Cite this

    Yeo, W. L., Chew, X., Smith, D. J., Chan, K. P., Sun, H., Zhao, H., Lim, Y. H., & Ang, E. L. (2017). Probing the molecular determinants of fluorinase specificity. Chemical Communications, 53(17), 2559-2562. https://doi.org/10.1039/c6cc09213f