Abstract
Fluorescence resonance energy transfer is a standard spectroscopic technique for measuring distances in the 10-75 angstrom range. This study extends the technique's sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule. The DNA was dried on amino-propyl-silanized glass. Near-field fluorescence images and emission spectra were taken on single DNA molecules labeled with donor only, with acceptor only, or with both donor and acceptor.
Original language | English (US) |
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Pages | 25 |
Number of pages | 1 |
State | Published - 1996 |
Externally published | Yes |
Event | Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS - Anaheim, CA, USA Duration: Jun 2 1996 → Jun 7 1996 |
Conference
Conference | Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS |
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City | Anaheim, CA, USA |
Period | 6/2/96 → 6/7/96 |
ASJC Scopus subject areas
- General Physics and Astronomy