Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor

S. Weiss, T. Ha, Th Enderle, D. F. Ogletree, P. R. Selvin, D. S. Chemla

Research output: Contribution to conferencePaper

Abstract

Fluorescence resonance energy transfer is a standard spectroscopic technique for measuring distances in the 10-75 angstrom range. This study extends the technique's sensitivity by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy was used to obtain simultaneous dual color imaging and emission spectra from a single pair of donor and acceptor fluorophores linked by a short (10 or 20 bases long) DNA molecule. The DNA was dried on amino-propyl-silanized glass. Near-field fluorescence images and emission spectra were taken on single DNA molecules labeled with donor only, with acceptor only, or with both donor and acceptor.

Original languageEnglish (US)
Number of pages1
StatePublished - Jan 1 1996
Externally publishedYes
EventProceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS - Anaheim, CA, USA
Duration: Jun 2 1996Jun 7 1996

Conference

ConferenceProceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS
CityAnaheim, CA, USA
Period6/2/966/7/96

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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    Weiss, S., Ha, T., Enderle, T., Ogletree, D. F., Selvin, P. R., & Chemla, D. S. (1996). Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Paper presented at Proceedings of the 1996 6th Quantum Electronics and Laser Science Conference, QELS, Anaheim, CA, USA, .