Probing genes and specific sequences on mechanically stretched single DNA molecules

Jason R. Taylor; Shuming Nie

Probing genes and specific sequences on mechanically stretched single DNA molecules

Jason R. Taylor
, Shuming Nie

Research output: Contribution to journal › Conference article › peer-review

Abstract

The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl₂, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.

Original language	English (US)
Pages (from-to)	9-10
Number of pages	2
Journal	American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume	50
State	Published - 2001
Externally published	Yes
Event	Proceedings of the 2001 Bioengineering Conference - Snowbird, UT, United States Duration: Jun 27 2001 → Jul 1 2001

ASJC Scopus subject areas

General Engineering

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title = "Probing genes and specific sequences on mechanically stretched single DNA molecules",

abstract = "The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl2, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.",

author = "Taylor, \{Jason R.\} and Shuming Nie",

year = "2001",

language = "English (US)",

volume = "50",

pages = "9--10",

journal = "American Society of Mechanical Engineers, Bioengineering Division (Publication) BED",

issn = "1071-6947",

note = "Proceedings of the 2001 Bioengineering Conference ; Conference date: 27-06-2001 Through 01-07-2001",

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AU - Taylor, Jason R.

AU - Nie, Shuming

PY - 2001

Y1 - 2001

N2 - The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl2, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.

AB - The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl2, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.

UR - https://www.scopus.com/pages/publications/0242694404

UR - https://www.scopus.com/pages/publications/0242694404#tab=citedBy

M3 - Conference article

AN - SCOPUS:0242694404

SN - 1071-6947

VL - 50

SP - 9

EP - 10

JO - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

JF - American Society of Mechanical Engineers, Bioengineering Division (Publication) BED

T2 - Proceedings of the 2001 Bioengineering Conference

Y2 - 27 June 2001 through 1 July 2001

ER -

Probing genes and specific sequences on mechanically stretched single DNA molecules

Abstract

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