The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl2, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.
|Original language||English (US)|
|Number of pages||2|
|Journal||American Society of Mechanical Engineers, Bioengineering Division (Publication) BED|
|State||Published - 2001|
|Event||Proceedings of the 2001 Bioengineering Conference - Snowbird, UT, United States|
Duration: Jun 27 2001 → Jul 1 2001
ASJC Scopus subject areas