Abstract
The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl2, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-10 |
| Number of pages | 2 |
| Journal | American Society of Mechanical Engineers, Bioengineering Division (Publication) BED |
| Volume | 50 |
| State | Published - 2001 |
| Externally published | Yes |
| Event | Proceedings of the 2001 Bioengineering Conference - Snowbird, UT, United States Duration: Jun 27 2001 → Jul 1 2001 |
ASJC Scopus subject areas
- General Engineering