Probing genes and specific sequences on mechanically stretched single DNA molecules

Jason R. Taylor, Shuming Nie

Research output: Contribution to journalConference articlepeer-review

Abstract

The use of nanometer-sized luminescent particles to study specific genes and sequences on single DNA molecules was presented. λ-DNA concatemers were prepared by incubating 100 ng λ-DNA (monomers) with five units of T4 DNA ligase in 50-mM Tris buffer containing 10-mM MgCl2, 1-m ATP, and 20 m BSA overnight at 15°C. The analysis showed the unique laminar flow profile that allow DNA molecules to be electrostatically attached to the surface.

Original languageEnglish (US)
Pages (from-to)9-10
Number of pages2
JournalAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume50
StatePublished - 2001
Externally publishedYes
EventProceedings of the 2001 Bioengineering Conference - Snowbird, UT, United States
Duration: Jun 27 2001Jul 1 2001

ASJC Scopus subject areas

  • Engineering(all)

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