Probing chemical messengers in a small nervous system with mass spectrometry

Linajun Li; Wayne P. Kelley; Stefan R. Pulver; Jonathan V Sweedler; Eve Marder

Probing chemical messengers in a small nervous system with mass spectrometry

Linajun Li, Wayne P. Kelley, Stefan R. Pulver, Jonathan V Sweedler, Eve Marder

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The array of mass spectrometry (MS)-based strategies to determine complement of peptide messengers in small neuronal networks was investigated using crustacean stomatogastric nervous system (STNS). Large number of neuromodulatory compounds were tested and identified based on physiological responses and immunostaining. The methods to examine peptide hormones in circulating hemolymph was also developed. The hybrid sample preparation approach was employed with centrifugation to remove high molecular weight species and microscale solid-phase extraction to desalt peptide hormones in hemolymph.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	393-394
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

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title = "Probing chemical messengers in a small nervous system with mass spectrometry",

abstract = "The array of mass spectrometry (MS)-based strategies to determine complement of peptide messengers in small neuronal networks was investigated using crustacean stomatogastric nervous system (STNS). Large number of neuromodulatory compounds were tested and identified based on physiological responses and immunostaining. The methods to examine peptide hormones in circulating hemolymph was also developed. The hybrid sample preparation approach was employed with centrifugation to remove high molecular weight species and microscale solid-phase extraction to desalt peptide hormones in hemolymph.",

author = "Linajun Li and Kelley, {Wayne P.} and Pulver, {Stefan R.} and Sweedler, {Jonathan V} and Eve Marder",

year = "2002",

language = "English (US)",

pages = "393--394",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - GEN

T1 - Probing chemical messengers in a small nervous system with mass spectrometry

AU - Li, Linajun

AU - Kelley, Wayne P.

AU - Pulver, Stefan R.

AU - Sweedler, Jonathan V

AU - Marder, Eve

PY - 2002

Y1 - 2002

N2 - The array of mass spectrometry (MS)-based strategies to determine complement of peptide messengers in small neuronal networks was investigated using crustacean stomatogastric nervous system (STNS). Large number of neuromodulatory compounds were tested and identified based on physiological responses and immunostaining. The methods to examine peptide hormones in circulating hemolymph was also developed. The hybrid sample preparation approach was employed with centrifugation to remove high molecular weight species and microscale solid-phase extraction to desalt peptide hormones in hemolymph.

AB - The array of mass spectrometry (MS)-based strategies to determine complement of peptide messengers in small neuronal networks was investigated using crustacean stomatogastric nervous system (STNS). Large number of neuromodulatory compounds were tested and identified based on physiological responses and immunostaining. The methods to examine peptide hormones in circulating hemolymph was also developed. The hybrid sample preparation approach was employed with centrifugation to remove high molecular weight species and microscale solid-phase extraction to desalt peptide hormones in hemolymph.

UR - http://www.scopus.com/inward/record.url?scp=2442642970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442642970&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442642970

SP - 393

EP - 394

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Probing chemical messengers in a small nervous system with mass spectrometry

Abstract

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