Probing chemical messengers in a small nervous system with mass spectrometry

Linajun Li, Wayne P. Kelley, Stefan R. Pulver, Jonathan V Sweedler, Eve Marder

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The array of mass spectrometry (MS)-based strategies to determine complement of peptide messengers in small neuronal networks was investigated using crustacean stomatogastric nervous system (STNS). Large number of neuromodulatory compounds were tested and identified based on physiological responses and immunostaining. The methods to examine peptide hormones in circulating hemolymph was also developed. The hybrid sample preparation approach was employed with centrifugation to remove high molecular weight species and microscale solid-phase extraction to desalt peptide hormones in hemolymph.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages393-394
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

ASJC Scopus subject areas

  • Spectroscopy

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