TY - JOUR
T1 - Principles and biophysical applications of lanthanide-based probes
AU - Selvin, Paul R.
PY - 2002
Y1 - 2002
N2 - Using luminescent lanthanides, instead of conventional fluorophores, as donor molecules in resonance energy transfer measurements offers many technical advantages and opens up a wide range of new applications. Advantages include farther measurable distances (∼100 Å) with greater accuracy, insensitivity to incomplete labeling, and the ability to use generic relatively large labels, when necessary. Applications highlighted include the study of ion channels in living cells, protein-protein interaction in cells, DNA-protein complexes, and high-throughput screening assays to measure peptide dimerization associated with DNA transcription factors and ligand-receptor interactions.
AB - Using luminescent lanthanides, instead of conventional fluorophores, as donor molecules in resonance energy transfer measurements offers many technical advantages and opens up a wide range of new applications. Advantages include farther measurable distances (∼100 Å) with greater accuracy, insensitivity to incomplete labeling, and the ability to use generic relatively large labels, when necessary. Applications highlighted include the study of ion channels in living cells, protein-protein interaction in cells, DNA-protein complexes, and high-throughput screening assays to measure peptide dimerization associated with DNA transcription factors and ligand-receptor interactions.
KW - Fluorescence resonance energy transfer
KW - Lanthanide
KW - Luminescence
UR - http://www.scopus.com/inward/record.url?scp=0036085517&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036085517&partnerID=8YFLogxK
U2 - 10.1146/annurev.biophys.31.101101.140927
DO - 10.1146/annurev.biophys.31.101101.140927
M3 - Review article
C2 - 11988471
AN - SCOPUS:0036085517
SN - 1056-8700
VL - 31
SP - 275
EP - 302
JO - Annual Review of Biophysics and Biomolecular Structure
JF - Annual Review of Biophysics and Biomolecular Structure
ER -