Primary and secondary structure of antifreeze peptides from arctic and antartic zoarcid fishes

Joseph D. Schrag, Chi-Hing C Cheng-De Vries, Maria Panico, Howard R. Morris, Arthur L. Deries

Research output: Contribution to journalArticle

Abstract

Antifreeze peptides were isolated from Rhigophial dearborni, an antartic eel pout, and Lycodes polaris, an arctic eel pout (both from the family Zoarcidae). The primary structures of two peptides, one from each species, were determined using a combination of Edman degradation and mass spectrometric techniques. The two sequences show a high degree of homology with nearly 80% of the residues being identical. These peptides are also homologous to antifreeze peptides from a third eel pout which inhabits the north Atlantic Ocean. The CD spectra of all of these peptides are also very similir. Unlike the antifreeze peptides of cottid fishes, the structures of antifreeze peptides from zoarcid fishes appear to be highly conserved, despite the large geographic distances which separate the different species. The zoarcid peptides also appear to have structures very different from other fish antifreezes.

Original languageEnglish (US)
Pages (from-to)357-370
Number of pages14
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume915
Issue number3
DOIs
StatePublished - Oct 15 1987

Fingerprint

Antifreeze Proteins
Eels
Fish
Fishes
Peptides
Atlantic Ocean
Degradation

Keywords

  • (Zoarcid fish)
  • Amino acid sequence
  • Antifreez peptide
  • Cold water fish
  • Ice crystal formation
  • Peptide structure

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Primary and secondary structure of antifreeze peptides from arctic and antartic zoarcid fishes. / Schrag, Joseph D.; Cheng-De Vries, Chi-Hing C; Panico, Maria; Morris, Howard R.; Deries, Arthur L.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 915, No. 3, 15.10.1987, p. 357-370.

Research output: Contribution to journalArticle

@article{085c04b0e80e4fffb0e3580d72ed0052,
title = "Primary and secondary structure of antifreeze peptides from arctic and antartic zoarcid fishes",
abstract = "Antifreeze peptides were isolated from Rhigophial dearborni, an antartic eel pout, and Lycodes polaris, an arctic eel pout (both from the family Zoarcidae). The primary structures of two peptides, one from each species, were determined using a combination of Edman degradation and mass spectrometric techniques. The two sequences show a high degree of homology with nearly 80{\%} of the residues being identical. These peptides are also homologous to antifreeze peptides from a third eel pout which inhabits the north Atlantic Ocean. The CD spectra of all of these peptides are also very similir. Unlike the antifreeze peptides of cottid fishes, the structures of antifreeze peptides from zoarcid fishes appear to be highly conserved, despite the large geographic distances which separate the different species. The zoarcid peptides also appear to have structures very different from other fish antifreezes.",
keywords = "(Zoarcid fish), Amino acid sequence, Antifreez peptide, Cold water fish, Ice crystal formation, Peptide structure",
author = "Schrag, {Joseph D.} and {Cheng-De Vries}, {Chi-Hing C} and Maria Panico and Morris, {Howard R.} and Deries, {Arthur L.}",
year = "1987",
month = "10",
day = "15",
doi = "10.1016/0167-4838(87)90021-5",
language = "English (US)",
volume = "915",
pages = "357--370",
journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",
issn = "1570-9639",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Primary and secondary structure of antifreeze peptides from arctic and antartic zoarcid fishes

AU - Schrag, Joseph D.

AU - Cheng-De Vries, Chi-Hing C

AU - Panico, Maria

AU - Morris, Howard R.

AU - Deries, Arthur L.

PY - 1987/10/15

Y1 - 1987/10/15

N2 - Antifreeze peptides were isolated from Rhigophial dearborni, an antartic eel pout, and Lycodes polaris, an arctic eel pout (both from the family Zoarcidae). The primary structures of two peptides, one from each species, were determined using a combination of Edman degradation and mass spectrometric techniques. The two sequences show a high degree of homology with nearly 80% of the residues being identical. These peptides are also homologous to antifreeze peptides from a third eel pout which inhabits the north Atlantic Ocean. The CD spectra of all of these peptides are also very similir. Unlike the antifreeze peptides of cottid fishes, the structures of antifreeze peptides from zoarcid fishes appear to be highly conserved, despite the large geographic distances which separate the different species. The zoarcid peptides also appear to have structures very different from other fish antifreezes.

AB - Antifreeze peptides were isolated from Rhigophial dearborni, an antartic eel pout, and Lycodes polaris, an arctic eel pout (both from the family Zoarcidae). The primary structures of two peptides, one from each species, were determined using a combination of Edman degradation and mass spectrometric techniques. The two sequences show a high degree of homology with nearly 80% of the residues being identical. These peptides are also homologous to antifreeze peptides from a third eel pout which inhabits the north Atlantic Ocean. The CD spectra of all of these peptides are also very similir. Unlike the antifreeze peptides of cottid fishes, the structures of antifreeze peptides from zoarcid fishes appear to be highly conserved, despite the large geographic distances which separate the different species. The zoarcid peptides also appear to have structures very different from other fish antifreezes.

KW - (Zoarcid fish)

KW - Amino acid sequence

KW - Antifreez peptide

KW - Cold water fish

KW - Ice crystal formation

KW - Peptide structure

UR - http://www.scopus.com/inward/record.url?scp=0023656535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023656535&partnerID=8YFLogxK

U2 - 10.1016/0167-4838(87)90021-5

DO - 10.1016/0167-4838(87)90021-5

M3 - Article

C2 - 3477289

AN - SCOPUS:0023656535

VL - 915

SP - 357

EP - 370

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

SN - 1570-9639

IS - 3

ER -