Prevalence of the K76T mutation in the pfcrt gene of Plasmodium falciparum among chloroquine responders in India

Sumiti Vinayak, Sukla Biswas, Vas Dev, Ashwani Kumar, M. A. Ansari, Y. D. Sharma

Research output: Contribution to journalArticlepeer-review

Abstract

Chloroquine-resistant Plasmodium falciparum needs to be monitored in the field for effective malaria control strategies. A point mutation K76T in the P. falciparum chloroquine resistance transporter (Pfcrt) protein has recently been proposed as a molecular marker for the faster detection of chloroquine-resistant falciparum malaria in field. We describe here the evaluation of this marker in Indian P. falciparum isolates. A total of 274 Indian P. falciparum isolates were analyzed for the K76T mutation. This mutation was detected in all the clinical isolates obtained from the in vivo chloroquine non-responders. But majority of the clinical isolates from chloroquine responders (71 of 74 patients, i.e. 96%) also harbored this mutation. The K76T mutation was indeed highly prevalent (91%) among 213 clinical isolates. There was a significant association between K76T mutation and the in vitro chloroquine response (P<0.05) but six isolates showed discordant results. In conclusion, the K76T mutation fails to differentiate majority of the chloroquine responders from that of the non-responders and thus will be of limited use in the field in India.

Original languageEnglish (US)
Pages (from-to)287-293
Number of pages7
JournalActa Tropica
Volume87
Issue number2
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Keywords

  • Chloroquine resistance
  • Malaria
  • P. falciparum
  • PCR
  • Pfcrt

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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