Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms

V. Srinivasan, H. M. Nam, L. T. Nguyen, B. Tamilselvam, S. E. Murinda, S. P. Oliver

Research output: Contribution to journalArticlepeer-review

Abstract

Antimicrobial resistance of Listeria monocytogenes (n = 38) isolated from the four dairy farms to 15 antimicrobial agents was evaluated. All 38 L. monocytogenes isolates from the four farms evaluated were resistant to more than one antimicrobial in different combinations. All L. monocytogenes isolates evaluated were resistant to cephalosporin C (minimum inhibitory concentration [MIC] ≥ 512 μg/mL), streptomycin (MIC ≥ 32) and trimethoprim (MIC ≥ 512). Most L. monocytogenes isolates were resistant to ampicillin (92%, MIC ≥ 2), rifampicin (84%, MIC ≥ 4), rifamycin (84%, MIC ≥ 4), and florfenicol (66%, MIC ≥ 32) and some were resistant to tetracycline (45%, MIC ≥ 16), penicillin G (40%, MIC ≥ 2) and chloramphenicol (32%, MIC ≥ 32). All L. monocytogenes isolates were susceptible to amoxicillin, erythromycin, gentamicin, kanamycin and vancomycin. Susceptibility of L. monocytogenes to the antimicrobials evaluated was quite consistent among the dairy farms evaluated. However, some variability in antimicrobial susceptibility among dairy farms was noted. Nineteen of 38 L. monocytogenes isolates contained more than one antimicrobial resistance gene sequence. A high frequency of floR (66%) was found in L. monocytogenes followed by penA (37%), strA (34%), tetA (32%), and sull (16%). Other tetracycline resistance genes (tetB, tetC, tetD, tetE, and tetG) and other antimicrobial resistance genes (cmlA, strB, aadA, sulI, vanA, vanB, ampC, ermB, ereA, and ereB) were not found in any of the L. monocytogenes isolates from the four dairy farms. Results of the present study demonstrated that L. monocytogenes isolated from the dairy farm environment were resistant to many antimicrobials and contained one or more antimicrobial resistance genes.

Original languageEnglish (US)
Pages (from-to)201-211
Number of pages11
JournalFoodborne Pathogens and Disease
Volume2
Issue number3
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

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