Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia

Hirohisa Mekata, Satoru Konnai, Martin Simuunza, Mwelwa Chembensofu, Rika Kano, William H. Witola, Mwase E. Tembo, Harrison Chitambo, Noboru Inoue, Misao Onuma, Kazuhiko Ohashi

Research output: Research - peer-reviewArticle

Abstract

The prevalence of trypanosome infections in tsetse flies, Glossina pallidipes, collected from Chiawa and Chakwenga in Zambia with endemic trypanosomosis was assessed by polymerase chain reaction (PCR). Out of the 550 G. pallidipes, 58 (10.5%) flies were found to harbor trypanosome DNA. Infection rates of tsetse with Trypanosoma vivax universal, Trypanosoma congolense savannah, T. congolense forest and T. congolense kilifi were 4.2% (23/550), 4.7% (26/550), 1.1% (6/550) and 1.6% (9/550), respectively. To determine the mammalian hosts of T. congolense and T. vivax infections from the tsetse flies, mammalian mitochondrion DNA of blood meal in these flies were analyzed by PCR and subsequent gene sequence analysis of the amplicons. Sequence analysis showed the presence of cytochrome b gene (cyt b) of 7 different mammalian species such as human, elephant, buffalo, goat, warthog, greater kudu and cattle. Goats which were main livestock in these areas were further examined to know the extent of its contribution in spreading the infection. We examined the prevalence of trypanosome infections in the domestic goat population in 6 settlements in Chiawa alone. Of the 86 goats sampled, 4 (4.6%), 5 (5.8%), 4 (4.6%) and 4 (4.6%) were positive for T. vivax universal, T. congolense savannah, forest and kilifi, respectively. These findings showed that the host-source of trypanosome infections in vector fly give a vital information about spread of infection. The result of this study will certainly contribute in elucidating more the epidemiology of trypanosomosis.

LanguageEnglish (US)
Pages923-928
Number of pages6
JournalJournal of Veterinary Medical Science
Volume70
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Tsetse Flies
Zambia
Trypanosomiasis
Diptera
Infection
Glossina pallidipes
infection
Trypanosoma congolense
Goats
goats
Trypanosoma vivax
Glossina
Sequence Analysis
Polymerase Chain Reaction
DNA
Genes
Forests
trypanosomiasis
savannas
sequence analysis

Keywords

  • Goat
  • Natural host
  • Trypanosome
  • Tsetse fly
  • Zambia

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia. / Mekata, Hirohisa; Konnai, Satoru; Simuunza, Martin; Chembensofu, Mwelwa; Kano, Rika; Witola, William H.; Tembo, Mwase E.; Chitambo, Harrison; Inoue, Noboru; Onuma, Misao; Ohashi, Kazuhiko.

In: Journal of Veterinary Medical Science, Vol. 70, No. 9, 09.2008, p. 923-928.

Research output: Research - peer-reviewArticle

Mekata, H, Konnai, S, Simuunza, M, Chembensofu, M, Kano, R, Witola, WH, Tembo, ME, Chitambo, H, Inoue, N, Onuma, M & Ohashi, K 2008, 'Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia' Journal of Veterinary Medical Science, vol 70, no. 9, pp. 923-928. DOI: 10.1292/jvms.70.923
Mekata, Hirohisa ; Konnai, Satoru ; Simuunza, Martin ; Chembensofu, Mwelwa ; Kano, Rika ; Witola, William H. ; Tembo, Mwase E. ; Chitambo, Harrison ; Inoue, Noboru ; Onuma, Misao ; Ohashi, Kazuhiko. / Prevalence and source of trypanosome infections in field-captured vector flies (Glossina pallidipes) in southeastern Zambia. In: Journal of Veterinary Medical Science. 2008 ; Vol. 70, No. 9. pp. 923-928
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