@article{cd6c31a46371462fb4d77cddea1ed3a6,
title = "Pressure-driven release of viral genome into a host nucleus is a mechanism leading to herpes infection",
abstract = "Many viruses previously have been shown to have pressurized genomes inside their viral protein shell, termed the capsid. This pressure results from the tight confinement of negatively charged viral nucleic acids inside the capsid. However, the relevance of capsid pressure to viral infection has not been demonstrated. In this work, we show that the internal DNA pressure of tens of atmospheres inside a herpesvirus capsid powers ejection of the viral genome into a host cell nucleus. To our knowledge, this provides the first demonstration of a pressure-dependent mechanism of viral genome penetration into a host nucleus, leading to infection of eukaryotic cells.",
author = "Alberto Brandariz-Nu{\~n}ez and Ting Liu and Te Du and Alex Evilevitch",
note = "We would like to thank Bernard Roizman for help with designing and conducting the pull-down assay experiments. We greatly acknowledge Fred Homa, Jamie Huffman, Roger Lipp\textbackslash{}u00E9, P\textbackslash{}u00E4ivi Ojala, Lindsay Smith, and Beate Sodeik, for sharing their viral strains, protocols, and important advice in preparation of this manuscript. We thank Scott Robinson for help with ultra-thin sectioning EM imaging. EM imaging was done at the Beckman Institute core facility at UIUC. We thank Glenn Fried and Austin Cyphersmith for help with SR-SIM imaging at the Carl R Woese Institute for Genomic Biology. Krista Freeman, Dong Li and Dave Bauer are acknowledged for help with the initial optimization of experimental conditions. Adri\textbackslash{}u00E1n Gonz\textbackslash{}u00E1lez Rodr\textbackslash{}u00EDguez is acknowledged for help with the calculation of pressures in herpesviruses. Funding for this work was provided by the National Science Foundation CHE-1507694 (to AE) and Swedish Research Council grants (VR) 621-2014-5537 and 349-2014\textbackslash{}u20133962 (to AE).",
year = "2019",
month = aug,
doi = "10.7554/eLife.47212",
language = "English (US)",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd",
}