Radioactive glycoproteins were prepared from the purified serum glycoproteins of antarctic fish by introducing tritium atoms (3H) into their carbohydrate moiety. The carbohydrate moiety is composed of disaccharides, β-D-galactosyl-(1→4) α-N-acetylgalactosamine, which accounts for 60% of the molecule. The hydroxyl groups (OH) at the carbon-6 positions of both sugar residues were oxidized with galactose oxidase to aldehyde groups and subsequently reduced to hydroxyl groups (O3H) with sodium borohydride-3H (NaB3H4). Under suitable conditions, the end products (tritiated glycoproteins) are highly radioactive and retain their original unique freezing point depressing activity.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 19 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology