Preparation of 3H-labeled serum glycoproteins from antarctic fish

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Abstract

Radioactive glycoproteins were prepared from the purified serum glycoproteins of antarctic fish by introducing tritium atoms (3H) into their carbohydrate moiety. The carbohydrate moiety is composed of disaccharides, β-D-galactosyl-(1→4) α-N-acetylgalactosamine, which accounts for 60% of the molecule. The hydroxyl groups (OH) at the carbon-6 positions of both sugar residues were oxidized with galactose oxidase to aldehyde groups and subsequently reduced to hydroxyl groups (O3H) with sodium borohydride-3H (NaB3H4). Under suitable conditions, the end products (tritiated glycoproteins) are highly radioactive and retain their original unique freezing point depressing activity.

Original languageEnglish (US)
Pages (from-to)1192-1196
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume59
Issue number4
DOIs
StatePublished - Aug 19 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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