Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy

Marcus D. Tuttle, Joseph M. Courtney, Alexander M. Barclay, Chad M. Rienstra

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Solid-state NMR spectroscopy (SSNMR) is an established and invaluable tool for the study of amyloid fibril structure with atomic-level detail. Optimization of the homogeneity and concentration of fibrils enhances the resolution and sensitivity of SSNMR spectra. Here, we present a fibrillization and fibril processing protocol, starting from purified monomeric α-synuclein, that enables the collection of highresolution SSNMR spectra suitable for site-specific structural analysis. This protocol does not rely on any special features of α-synuclein and should be generalizable to any other amyloid protein.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages173-183
Number of pages11
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1345
ISSN (Print)1064-3745

Fingerprint

Synucleins
Amyloid
Magnetic Resonance Spectroscopy
Amyloidogenic Proteins

Keywords

  • Amyloid fibrils
  • Fibrillization
  • Size exclusion chromatography
  • Solid-state NMR
  • α-synuclein

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Tuttle, M. D., Courtney, J. M., Barclay, A. M., & Rienstra, C. M. (2016). Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy. In Methods in Molecular Biology (pp. 173-183). (Methods in Molecular Biology; Vol. 1345). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2978-8_11

Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy. / Tuttle, Marcus D.; Courtney, Joseph M.; Barclay, Alexander M.; Rienstra, Chad M.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 173-183 (Methods in Molecular Biology; Vol. 1345).

Research output: Chapter in Book/Report/Conference proceedingChapter

Tuttle, MD, Courtney, JM, Barclay, AM & Rienstra, CM 2016, Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1345, Humana Press Inc., pp. 173-183. https://doi.org/10.1007/978-1-4939-2978-8_11
Tuttle MD, Courtney JM, Barclay AM, Rienstra CM. Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 173-183. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2978-8_11
Tuttle, Marcus D. ; Courtney, Joseph M. ; Barclay, Alexander M. ; Rienstra, Chad M. / Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 173-183 (Methods in Molecular Biology).
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