Prenatal exposure to di-(2-ethylhexyl) phthalate (DEHP) affects reproductive outcomes in female mice

Sarah Niermann, Saniya Rattan, Emily Brehm, Jodi A. Flaws

Research output: Contribution to journalArticlepeer-review

Abstract

This study tested the hypothesis that prenatal DEHP exposure affects female reproduction. To test this hypothesis, pregnant female CD-1 mice were orally dosed daily with tocopherol-stripped corn oil (vehicle control) or DEHP (20. μg/kg/day-750. mg/kg/day) from gestation day 11-birth. Pups were counted, weighed, and sexed at birth, ovaries were subjected to evaluations of follicle numbers on postnatal days (PNDs) 8 and 21, and fertility was evaluated at 3-9 months. The results indicate that prenatal DEHP exposure increased male-to-female ratio compared to controls. Prenatal DEHP exposure also increased preantral follicle numbers at PND 21 compared to controls. Further, 22.2% of the 20. μg/kg/day treated animals took longer than 5 days to get pregnant at 3 months and 28.6% of the 750. mg/kg/day treated animals lost some of their pups at 6 months. Thus, prenatal DEHP exposure alters F1 sex ratio, increases preantral follicle numbers, and causes some breeding abnormalities.

Original languageEnglish (US)
Pages (from-to)23-32
Number of pages10
JournalReproductive Toxicology
Volume53
DOIs
StatePublished - Jun 1 2015

Keywords

  • DEHP
  • Estrous cyclicity
  • Female reproduction
  • Ovary
  • Phthalate
  • Sex ratio

ASJC Scopus subject areas

  • Toxicology

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