Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay

L. Zsak; M. V. Borca; G. R. Risatti; A. Zsak; R. A. French; Z. Lu; G. F. Kutish; J. G. Neilan; J. D. Callahan; W. M. Nelson; D. L. Rock

doi:10.1128/JCM.43.1.112-119.2005

Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay

L. Zsak, M. V. Borca, G. R. Risatti, A. Zsak, R. A. French, Z. Lu, G. F. Kutish, J. G. Neilan, J. D. Callahan, W. M. Nelson, D. L. Rock

Research output: Contribution to journal › Article › peer-review

Abstract

A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

Original language	English (US)
Pages (from-to)	112-119
Number of pages	8
Journal	Journal of Clinical Microbiology
Volume	43
Issue number	1
DOIs	https://doi.org/10.1128/JCM.43.1.112-119.2005
State	Published - Jan 2005
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

Online availability

10.1128/JCM.43.1.112-119.2005

Library availability

Discover UIUC Full Text

Cite this

@article{08fc3588c7c14ff2b2d0e0d33a6c757f,

title = "Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay",

abstract = "A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.",

author = "L. Zsak and Borca, {M. V.} and Risatti, {G. R.} and A. Zsak and French, {R. A.} and Z. Lu and Kutish, {G. F.} and Neilan, {J. G.} and Callahan, {J. D.} and Nelson, {W. M.} and Rock, {D. L.}",

year = "2005",

month = jan,

doi = "10.1128/JCM.43.1.112-119.2005",

language = "English (US)",

volume = "43",

pages = "112--119",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "1",

}

TY - JOUR

T1 - Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay

AU - Zsak, L.

AU - Borca, M. V.

AU - Risatti, G. R.

AU - Zsak, A.

AU - French, R. A.

AU - Lu, Z.

AU - Kutish, G. F.

AU - Neilan, J. G.

AU - Callahan, J. D.

AU - Nelson, W. M.

AU - Rock, D. L.

PY - 2005/1

Y1 - 2005/1

N2 - A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

AB - A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

UR - http://www.scopus.com/inward/record.url?scp=19944426797&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=19944426797&partnerID=8YFLogxK

U2 - 10.1128/JCM.43.1.112-119.2005

DO - 10.1128/JCM.43.1.112-119.2005

M3 - Article

C2 - 15634958

AN - SCOPUS:19944426797

SN - 0095-1137

VL - 43

SP - 112

EP - 119

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 1

ER -

Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this