Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay

L. Zsak, M. V. Borca, G. R. Risatti, A. Zsak, R. A. French, Z. Lu, G. F. Kutish, J. G. Neilan, J. D. Callahan, W. M. Nelson, D. L. Rock

Research output: Contribution to journalArticlepeer-review

Abstract

A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.

Original languageEnglish (US)
Pages (from-to)112-119
Number of pages8
JournalJournal of Clinical Microbiology
Volume43
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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