@article{357c90a91d23463ab9d41b211ef1d61f,
title = "Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator",
abstract = "One-photon fluorescent imaging of calcium signals can capture the activity of hundreds of neurons across large fields of view but suffers from crosstalk from neuropil. Shemesh et al. engineer cell-body-targeted variants of fluorescent calcium indicators and show in mice and zebrafish that artifactual spikes and correlations are greatly reduced.",
keywords = "calcium imaging, correlation, crosstalk, GCaMP6, GCaMP7, in vivo imaging, microscopy, neuropil contamination, soma-targeting, two-photon microscopy",
author = "Shemesh, {Or A.} and Changyang Linghu and Piatkevich, {Kiryl D.} and Daniel Goodwin and Celiker, {Orhan Tunc} and Gritton, {Howard J.} and Romano, {Michael F.} and Ruixuan Gao and Yu, {Chih Chieh (Jay)} and Tseng, {Hua An} and Seth Bensussen and Sujatha Narayan and Yang, {Chao Tsung} and Limor Freifeld and Siciliano, {Cody A.} and Ishan Gupta and Joyce Wang and Nikita Pak and Yoon, {Young Gyu} and Ullmann, {Jeremy F.P.} and Burcu Guner-Ataman and Habiba Noamany and Sheinkopf, {Zoe R.} and Park, {Won Min} and Shoh Asano and Keating, {Amy E.} and Trimmer, {James S.} and Jacob Reimer and Tolias, {Andreas S.} and Bear, {Mark F.} and Tye, {Kay M.} and Xue Han and Ahrens, {Misha B.} and Boyden, {Edward S.}",
note = "Funding Information: We thank Eftychios Pnevmatikakis and Andrea Giovannucci from the Flatiron Institute at the Simons Foundation for useful discussions about computational methods for neuropil contamination reduction and for their active development of NormCorre and CaImAn. K.M.T. was a New York Stem Cell Foundation Robertson Investigator and McKnight Scholar, and this work was supported by funding from R01-MH102441 ( NIMH ) and Pioneer Award DP1-AT009925 ( NCCIH ). C.A.S. was supported by NIH grants F32 MH111216 (NIMH) and K99 DA045103 (NIDA), and a NARSAD Young Investigator Award ( Brain and Behavior Research Foundation ). J.S.T. and E.S.B. were supported by NIH U24 NS109113 . E.S.B. was supported by Lisa Yang ; John Doerr ; NIH 1R01DA045549 , 1R01MH114031 , and RF1NS113287 ; the HHMI-Simons Faculty Scholars Program; Human Frontier Science Program RGP0015/2016 ; U.S. Army Research Laboratory and U.S. Army Research Office under contract/grant W911NF1510548 ; U.S-Israel Binational Science Foundation grant 2014509 ; NSF 1848029 and 1734870 ; NIH 2R01DA029639 ; NSF CBET 1344219 ; the MIT Media Lab ; the Open Philanthropy Project ; NIH 1R24MH106075 , R44EB021054 , and 1R01GM104948 ; and NIH Director{\textquoteright}s Pioneer Award 1DP1NS087724 . J.T. acknowledges NIH grant U01 NS099714 . J.W. is supported by the NSF Graduate Research Fellowship grant 174530 . Publisher Copyright: {\textcopyright} 2020 Elsevier Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = aug,
day = "5",
doi = "10.1016/j.neuron.2020.05.029",
language = "English (US)",
volume = "107",
pages = "P470--486.e11",
journal = "Journal of Cleaner Production",
issn = "0959-6526",
publisher = "Elsevier Limited",
number = "3",
}