Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator

Or A. Shemesh, Changyang Linghu, Kiryl D. Piatkevich, Daniel Goodwin, Orhan Tunc Celiker, Howard J. Gritton, Michael F. Romano, Ruixuan Gao, Chih Chieh (Jay) Yu, Hua An Tseng, Seth Bensussen, Sujatha Narayan, Chao Tsung Yang, Limor Freifeld, Cody A. Siciliano, Ishan Gupta, Joyce Wang, Nikita Pak, Young Gyu Yoon, Jeremy F.P. UllmannBurcu Guner-Ataman, Habiba Noamany, Zoe R. Sheinkopf, Won Min Park, Shoh Asano, Amy E. Keating, James S. Trimmer, Jacob Reimer, Andreas S. Tolias, Mark F. Bear, Kay M. Tye, Xue Han, Misha B. Ahrens, Edward S. Boyden

Research output: Contribution to journalArticlepeer-review


One-photon fluorescent imaging of calcium signals can capture the activity of hundreds of neurons across large fields of view but suffers from crosstalk from neuropil. Shemesh et al. engineer cell-body-targeted variants of fluorescent calcium indicators and show in mice and zebrafish that artifactual spikes and correlations are greatly reduced.

Original languageEnglish (US)
Pages (from-to)P470-486.e11
JournalJournal of Cleaner Production
Issue number3
StatePublished - Aug 5 2020
Externally publishedYes


  • calcium imaging
  • correlation
  • crosstalk
  • GCaMP6
  • GCaMP7
  • in vivo imaging
  • microscopy
  • neuropil contamination
  • soma-targeting
  • two-photon microscopy

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • General Environmental Science
  • Strategy and Management
  • Industrial and Manufacturing Engineering


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