Precise characterization of human histones in the H2A gene family by top down mass spectrometry

Michael T. Boyne, James J. Pesavento, Craig A. Mizzen, Neil L. Kelleher

Research output: Contribution to journalArticle

Abstract

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

Original languageEnglish (US)
Pages (from-to)248-253
Number of pages6
JournalJournal of Proteome Research
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2006

Keywords

  • Cell cycle
  • Chromatin
  • Electron capture dissociation (ECD)
  • Fourier transform mass spectrometry (FTMS)
  • Histone H2A
  • Histone code
  • Post-translational modification (PTM)
  • SILAC
  • Top Down
  • Ubiquitination

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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