Precise characterization of human histones in the H2A gene family by top down mass spectrometry

Michael T. Boyne, James J. Pesavento, Craig A. Mizzen, Neil L. Kelleher

Research output: Contribution to journalArticlepeer-review

Abstract

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

Original languageEnglish (US)
Pages (from-to)248-253
Number of pages6
JournalJournal of Proteome Research
Volume5
Issue number2
DOIs
StatePublished - Feb 2006

Keywords

  • Cell cycle
  • Chromatin
  • Electron capture dissociation (ECD)
  • Fourier transform mass spectrometry (FTMS)
  • Histone H2A
  • Histone code
  • Post-translational modification (PTM)
  • SILAC
  • Top Down
  • Ubiquitination

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Fingerprint

Dive into the research topics of 'Precise characterization of human histones in the H2A gene family by top down mass spectrometry'. Together they form a unique fingerprint.

Cite this