Precise characterization of human histones in the H2A gene family by top down mass spectrometry

Michael T. Boyne, James J. Pesavento, Craig Andrew Mizzen, Neil L. Kelleher

Research output: Contribution to journalArticle

Abstract

Top Down analysis revealed that at least fourteen genes encoding histone H2A are coexpressed in HeLa cells. Characterization of these species revealed that all except H2A.Z and H2A.F/Z were α-N-acetylated, H2A.O and H2A.C,D,I,N,P were the most abundant, and those exceeding ∼10% abundance lacked post-translational modifications. This unequivocal identification of H2A forms illustrates the advantages of Top Down Mass Spectrometry and provides a global perspective of H2A regulation through the cell cycle.

Original languageEnglish (US)
Pages (from-to)248-253
Number of pages6
JournalJournal of Proteome Research
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2006

Fingerprint

Gene encoding
Post Translational Protein Processing
HeLa Cells
Histones
Mass spectrometry
Mass Spectrometry
Cell Cycle
Genes
Cells

Keywords

  • Cell cycle
  • Chromatin
  • Electron capture dissociation (ECD)
  • Fourier transform mass spectrometry (FTMS)
  • Histone H2A
  • Histone code
  • Post-translational modification (PTM)
  • SILAC
  • Top Down
  • Ubiquitination

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Precise characterization of human histones in the H2A gene family by top down mass spectrometry. / Boyne, Michael T.; Pesavento, James J.; Mizzen, Craig Andrew; Kelleher, Neil L.

In: Journal of Proteome Research, Vol. 5, No. 2, 01.02.2006, p. 248-253.

Research output: Contribution to journalArticle

Boyne, Michael T. ; Pesavento, James J. ; Mizzen, Craig Andrew ; Kelleher, Neil L. / Precise characterization of human histones in the H2A gene family by top down mass spectrometry. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 2. pp. 248-253.
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