TY - JOUR
T1 - Prebiotics and bioactive milk fractions affect gut development, microbiota, and neurotransmitter expression in piglets
AU - Berding, Kirsten
AU - Wang, Mei
AU - Monaco, Marcia H.
AU - Alexander, Lindsey S.
AU - Mudd, Austin T.
AU - Chichlowski, Maciej
AU - Waworuntu, Rosaline V.
AU - Berg, Brian M.
AU - Miller, Michael J.
AU - Dilger, Ryan N.
AU - Donovan, Sharon M.
N1 - Publisher Copyright:
Copyright © ESPGHAL and NASPGHAN. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Objective: This study tested the hypothesis that the addition of prebiotics and 2 functional milk ingredients to infant formula would maintain normal growth and gut development, and modify microbiota composition and neurotransmitter gene expression in neonatal piglets. Methods: Two-day-old male piglets (n=24) were fed formula (CONT) or formula with polydextrose (1.2 g/100 g diet), galactooligosaccharides (3.5 g/ 100 g diet), bovine lactoferrin (0.3 g/100 g diet), and milk fat globule membrane-10 (2.5 g/100 g diet) (TEST) for 30 days. On study day 31, intestinal samples, ileal and colonic contents, and feces were collected. Intestinal histomorphology, disaccharidase activity, serotonin (5'HT), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) were measured. Gut microbiota composition was assessed by pyrosequencing of the V3-V5 regions of 16S rRNA and quantitative polymerase chain reaction. Results: Body weight of piglets on TEST was greater (P≤0.05) than CONT on days 17 to 30. Both groups displayed growth patterns within the range observed for sow-reared pigs. TEST piglets had greater jejunal lactase (P=0.03) and higher (P=0.003) ileal VIP expression. TEST piglets tended to have greater (P=0.09) sucrase activity, longer (P=0.08) ileal villi, and greater (P=0.06) duodenal TH expression. Microbial communities of TEST piglets differed fromCONT in ascending colon (AC, P=0.001) and feces (P≤0.05). CONT piglets had greater relative abundances of Mogibacterium, Collinsella, Klebsiella, Escherichia/Shigella, Eubacterium, and Roseburia compared with TEST piglets in AC. In feces, CONT piglets harbored lower (P≤0.05) proportions of Parabacteroides, Clostridium IV, Lutispora, and Sutterella than TEST piglets. Conclusions: A mixture of bioactive ingredients improved weight gain and gut maturation, modulated colonic and fecal microbial composition, and reduced the proportions of opportunistic pathogens.
AB - Objective: This study tested the hypothesis that the addition of prebiotics and 2 functional milk ingredients to infant formula would maintain normal growth and gut development, and modify microbiota composition and neurotransmitter gene expression in neonatal piglets. Methods: Two-day-old male piglets (n=24) were fed formula (CONT) or formula with polydextrose (1.2 g/100 g diet), galactooligosaccharides (3.5 g/ 100 g diet), bovine lactoferrin (0.3 g/100 g diet), and milk fat globule membrane-10 (2.5 g/100 g diet) (TEST) for 30 days. On study day 31, intestinal samples, ileal and colonic contents, and feces were collected. Intestinal histomorphology, disaccharidase activity, serotonin (5'HT), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) were measured. Gut microbiota composition was assessed by pyrosequencing of the V3-V5 regions of 16S rRNA and quantitative polymerase chain reaction. Results: Body weight of piglets on TEST was greater (P≤0.05) than CONT on days 17 to 30. Both groups displayed growth patterns within the range observed for sow-reared pigs. TEST piglets had greater jejunal lactase (P=0.03) and higher (P=0.003) ileal VIP expression. TEST piglets tended to have greater (P=0.09) sucrase activity, longer (P=0.08) ileal villi, and greater (P=0.06) duodenal TH expression. Microbial communities of TEST piglets differed fromCONT in ascending colon (AC, P=0.001) and feces (P≤0.05). CONT piglets had greater relative abundances of Mogibacterium, Collinsella, Klebsiella, Escherichia/Shigella, Eubacterium, and Roseburia compared with TEST piglets in AC. In feces, CONT piglets harbored lower (P≤0.05) proportions of Parabacteroides, Clostridium IV, Lutispora, and Sutterella than TEST piglets. Conclusions: A mixture of bioactive ingredients improved weight gain and gut maturation, modulated colonic and fecal microbial composition, and reduced the proportions of opportunistic pathogens.
KW - Disaccharidase
KW - Gut maturation
KW - Microbiota
KW - Serotonin
KW - Tyrosine hydroxylase
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U2 - 10.1097/MPG.0000000000001200
DO - 10.1097/MPG.0000000000001200
M3 - Article
C2 - 27031373
AN - SCOPUS:84962052996
SN - 0277-2116
VL - 63
SP - 688
EP - 697
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
IS - 6
ER -