Prebiotics and bioactive milk fractions affect gut development, microbiota, and neurotransmitter expression in piglets

Kirsten Berding, Mei Wang, Marcia Helena Siegel, Lindsey S. Alexander, Austin T. Mudd, Maciej Chichlowski, Rosaline V. Waworuntu, Brian M. Berg, Michael J Miller, Ryan Neil Dilger, Sharon M Donovan

Research output: Contribution to journalArticle

Abstract

Objective: This study tested the hypothesis that the addition of prebiotics and 2 functional milk ingredients to infant formula would maintain normal growth and gut development, and modify microbiota composition and neurotransmitter gene expression in neonatal piglets. Methods: Two-day-old male piglets (n=24) were fed formula (CONT) or formula with polydextrose (1.2 g/100 g diet), galactooligosaccharides (3.5 g/ 100 g diet), bovine lactoferrin (0.3 g/100 g diet), and milk fat globule membrane-10 (2.5 g/100 g diet) (TEST) for 30 days. On study day 31, intestinal samples, ileal and colonic contents, and feces were collected. Intestinal histomorphology, disaccharidase activity, serotonin (5'HT), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) were measured. Gut microbiota composition was assessed by pyrosequencing of the V3-V5 regions of 16S rRNA and quantitative polymerase chain reaction. Results: Body weight of piglets on TEST was greater (P≤0.05) than CONT on days 17 to 30. Both groups displayed growth patterns within the range observed for sow-reared pigs. TEST piglets had greater jejunal lactase (P=0.03) and higher (P=0.003) ileal VIP expression. TEST piglets tended to have greater (P=0.09) sucrase activity, longer (P=0.08) ileal villi, and greater (P=0.06) duodenal TH expression. Microbial communities of TEST piglets differed fromCONT in ascending colon (AC, P=0.001) and feces (P≤0.05). CONT piglets had greater relative abundances of Mogibacterium, Collinsella, Klebsiella, Escherichia/Shigella, Eubacterium, and Roseburia compared with TEST piglets in AC. In feces, CONT piglets harbored lower (P≤0.05) proportions of Parabacteroides, Clostridium IV, Lutispora, and Sutterella than TEST piglets. Conclusions: A mixture of bioactive ingredients improved weight gain and gut maturation, modulated colonic and fecal microbial composition, and reduced the proportions of opportunistic pathogens.

Original languageEnglish (US)
Pages (from-to)688-697
Number of pages10
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume63
Issue number6
DOIs
StatePublished - Jan 1 2016

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Prebiotics
Neurotransmitter Agents
Milk
Feces
Diet
polydextrose
Vasoactive Intestinal Peptide
Tyrosine 3-Monooxygenase
Serotonin
Disaccharidases
Eubacterium
Sucrase
Lactase
Escherichia
Ascending Colon
Infant Formula
Lactoferrin
Shigella
Klebsiella
Clostridium

Keywords

  • Disaccharidase
  • Gut maturation
  • Microbiota
  • Serotonin
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

Cite this

Prebiotics and bioactive milk fractions affect gut development, microbiota, and neurotransmitter expression in piglets. / Berding, Kirsten; Wang, Mei; Siegel, Marcia Helena; Alexander, Lindsey S.; Mudd, Austin T.; Chichlowski, Maciej; Waworuntu, Rosaline V.; Berg, Brian M.; Miller, Michael J; Dilger, Ryan Neil; Donovan, Sharon M.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 63, No. 6, 01.01.2016, p. 688-697.

Research output: Contribution to journalArticle

Berding, Kirsten ; Wang, Mei ; Siegel, Marcia Helena ; Alexander, Lindsey S. ; Mudd, Austin T. ; Chichlowski, Maciej ; Waworuntu, Rosaline V. ; Berg, Brian M. ; Miller, Michael J ; Dilger, Ryan Neil ; Donovan, Sharon M. / Prebiotics and bioactive milk fractions affect gut development, microbiota, and neurotransmitter expression in piglets. In: Journal of Pediatric Gastroenterology and Nutrition. 2016 ; Vol. 63, No. 6. pp. 688-697.
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abstract = "Objective: This study tested the hypothesis that the addition of prebiotics and 2 functional milk ingredients to infant formula would maintain normal growth and gut development, and modify microbiota composition and neurotransmitter gene expression in neonatal piglets. Methods: Two-day-old male piglets (n=24) were fed formula (CONT) or formula with polydextrose (1.2 g/100 g diet), galactooligosaccharides (3.5 g/ 100 g diet), bovine lactoferrin (0.3 g/100 g diet), and milk fat globule membrane-10 (2.5 g/100 g diet) (TEST) for 30 days. On study day 31, intestinal samples, ileal and colonic contents, and feces were collected. Intestinal histomorphology, disaccharidase activity, serotonin (5'HT), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) were measured. Gut microbiota composition was assessed by pyrosequencing of the V3-V5 regions of 16S rRNA and quantitative polymerase chain reaction. Results: Body weight of piglets on TEST was greater (P≤0.05) than CONT on days 17 to 30. Both groups displayed growth patterns within the range observed for sow-reared pigs. TEST piglets had greater jejunal lactase (P=0.03) and higher (P=0.003) ileal VIP expression. TEST piglets tended to have greater (P=0.09) sucrase activity, longer (P=0.08) ileal villi, and greater (P=0.06) duodenal TH expression. Microbial communities of TEST piglets differed fromCONT in ascending colon (AC, P=0.001) and feces (P≤0.05). CONT piglets had greater relative abundances of Mogibacterium, Collinsella, Klebsiella, Escherichia/Shigella, Eubacterium, and Roseburia compared with TEST piglets in AC. In feces, CONT piglets harbored lower (P≤0.05) proportions of Parabacteroides, Clostridium IV, Lutispora, and Sutterella than TEST piglets. Conclusions: A mixture of bioactive ingredients improved weight gain and gut maturation, modulated colonic and fecal microbial composition, and reduced the proportions of opportunistic pathogens.",
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AU - Berding, Kirsten

AU - Wang, Mei

AU - Siegel, Marcia Helena

AU - Alexander, Lindsey S.

AU - Mudd, Austin T.

AU - Chichlowski, Maciej

AU - Waworuntu, Rosaline V.

AU - Berg, Brian M.

AU - Miller, Michael J

AU - Dilger, Ryan Neil

AU - Donovan, Sharon M

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N2 - Objective: This study tested the hypothesis that the addition of prebiotics and 2 functional milk ingredients to infant formula would maintain normal growth and gut development, and modify microbiota composition and neurotransmitter gene expression in neonatal piglets. Methods: Two-day-old male piglets (n=24) were fed formula (CONT) or formula with polydextrose (1.2 g/100 g diet), galactooligosaccharides (3.5 g/ 100 g diet), bovine lactoferrin (0.3 g/100 g diet), and milk fat globule membrane-10 (2.5 g/100 g diet) (TEST) for 30 days. On study day 31, intestinal samples, ileal and colonic contents, and feces were collected. Intestinal histomorphology, disaccharidase activity, serotonin (5'HT), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) were measured. Gut microbiota composition was assessed by pyrosequencing of the V3-V5 regions of 16S rRNA and quantitative polymerase chain reaction. Results: Body weight of piglets on TEST was greater (P≤0.05) than CONT on days 17 to 30. Both groups displayed growth patterns within the range observed for sow-reared pigs. TEST piglets had greater jejunal lactase (P=0.03) and higher (P=0.003) ileal VIP expression. TEST piglets tended to have greater (P=0.09) sucrase activity, longer (P=0.08) ileal villi, and greater (P=0.06) duodenal TH expression. Microbial communities of TEST piglets differed fromCONT in ascending colon (AC, P=0.001) and feces (P≤0.05). CONT piglets had greater relative abundances of Mogibacterium, Collinsella, Klebsiella, Escherichia/Shigella, Eubacterium, and Roseburia compared with TEST piglets in AC. In feces, CONT piglets harbored lower (P≤0.05) proportions of Parabacteroides, Clostridium IV, Lutispora, and Sutterella than TEST piglets. Conclusions: A mixture of bioactive ingredients improved weight gain and gut maturation, modulated colonic and fecal microbial composition, and reduced the proportions of opportunistic pathogens.

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KW - Gut maturation

KW - Microbiota

KW - Serotonin

KW - Tyrosine hydroxylase

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