Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging

Frances N. Keith, Rohith K. Reddy, Rohit Bhargava

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fourier transform infrared (FT-IR) spectroscopic imaging is an emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. We demonstrate a new concept in obtaining high fidelity data using commercial array detectors coupled to a microscope and Michelson interferometer. Next, we apply the developed technique to rapidly provide automated histopathologic information for breast cancer. Traditionally, disease diagnoses are based on optical examinations of stained tissue and involve a skilled recognition of morphological patterns of specific cell types (histopathology). Consequently, histopathologic determinations are a time consuming, subjective process with innate intra- and inter-operator variability. Utilizing endogenous molecular contrast inherent in vibrational spectra, specially designed tissue microarrays and pattern recognition of specific biochemical features, we report an integrated algorithm for automated classifications. The developed protocol is objective, statistically significant and, being compatible with current tissue processing procedures, holds potential for routine clinical diagnoses. We first demonstrate that the classification of tissue type (histology) can be accomplished in a manner that is robust and rigorous. Since data quality and classifier performance are linked, we quantify the relationship through our analysis model. Last, we demonstrate the application of the minimum noise fraction (MNF) transform to improve tissue segmentation.

Original languageEnglish (US)
Title of host publicationBiomedical Optical Spectroscopy
DOIs
StatePublished - Apr 21 2008
EventBiomedical Optical Spectroscopy - San Jose, CA, United States
Duration: Jan 19 2008Jan 23 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6853
ISSN (Print)1605-7422

Other

OtherBiomedical Optical Spectroscopy
CountryUnited States
CitySan Jose, CA
Period1/19/081/23/08

Fingerprint

Fourier transforms
Tissue
Infrared radiation
Imaging techniques
Michelson interferometers
Histology
Vibrational spectra
Microarrays
Pattern recognition
Optical microscopy
Microscopes
Classifiers
Spectroscopy
Detectors
Processing

Keywords

  • Breast cancer
  • Diagnostics
  • FT-IR spectroscopy
  • Histopathology
  • Hyperspectral
  • Imaging
  • MNF transform

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Keith, F. N., Reddy, R. K., & Bhargava, R. (2008). Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging. In Biomedical Optical Spectroscopy [685306] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6853). https://doi.org/10.1117/12.762468

Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging. / Keith, Frances N.; Reddy, Rohith K.; Bhargava, Rohit.

Biomedical Optical Spectroscopy. 2008. 685306 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6853).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Keith, FN, Reddy, RK & Bhargava, R 2008, Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging. in Biomedical Optical Spectroscopy., 685306, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6853, Biomedical Optical Spectroscopy, San Jose, CA, United States, 1/19/08. https://doi.org/10.1117/12.762468
Keith FN, Reddy RK, Bhargava R. Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging. In Biomedical Optical Spectroscopy. 2008. 685306. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.762468
Keith, Frances N. ; Reddy, Rohith K. ; Bhargava, Rohit. / Practical protocols for fast histopathology by Fourier transform infrared spectroscopic imaging. Biomedical Optical Spectroscopy. 2008. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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