Postpartal subclinical endometritis alters transcriptome profiles in liver and adipose tissue of dairy cows

Haji Akbar, Felipe C. Cardoso, Susanne Meier, Christopher Burke, Scott McDougall, Murray Mitchell, Caroline Walker, Sandra L. Rodriguez-Zas, Robin E. Everts, Harris A. Lewin, John R. Roche, Juan J. Loor

Research output: Contribution to journalArticle

Abstract

Transcriptome alterations in liver and adipose tissue of cows with subclinical endometritis (SCE) at 29 d postpartum were evaluated. Bioinformatics analysis was performed using the Dynamic Impact Approach by means of KEGG and DAVID databases. Milk production, blood metabo-lites (non-esterified fatty acids, magnesium), and disease biomarkers (albumin, aspartate aminotransferase) did not differ greatly between healthy and SCE cows. In liver tissue of cows with SCE, alterations in gene expression revealed an activation of complement and coagulation cascade, steroid hormone biosynthesis, apoptosis, inflammation, oxidative stress, MAPK signaling, and the formation of fibrinogen complex. Bioinformatics analysis also revealed an inhibition of vitamin B3 and B6 metabolism with SCE. In adipose, the most activated pathways by SCE were nicotinate and nicotinamide metabolism, long-chain fatty acid transport, oxidative phosphorylation, inflammation, T cell and B cell receptor signaling, and mTOR signaling. Results indicate that SCE in dairy cattle during early lactation induces molecular alterations in liver and adipose tissue indicative of immune activation and cellular stress.

Original languageEnglish (US)
Pages (from-to)45-63
Number of pages19
JournalBioinformatics and Biology Insights
Volume8
DOIs
StatePublished - Feb 19 2014

Keywords

  • Adipose
  • Cow genomics
  • Liver
  • Uterine infection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Computational Mathematics
  • Applied Mathematics

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