Abstract
Aim: We investigate how postweaning diet may modify the epigenetic landscape to meet metabolic demands later in life. Methods: Sprague-Dawley rats were exposed to a high-fat (HF) diet during gestation and lactation. At weaning, male offspring were placed either on an HF diet (HF/HF) or a control diet (HF/C). Methylation-dependent immunoprecipitation sequencing and methylation-sensitive restriction enzyme sequencing were used to quantify hepatic DNA methylation. Results: Out of the 3966 identified differentially methylated regions, 37% were mapped to gene bodies while 6% fell within promoter or downstream regions. Differentially methylated genes were clustered in the type II diabetes mellitus and the adipocytokine signaling pathways. Conclusion: Our results indicate that compared with a lifelong HF diet, offspring exposed to a new postweaning control diet are able to remodel the hepatic epigenome, emphasizing the dynamic nature of the methylome even after early life.
Original language | English (US) |
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Pages (from-to) | 1387-1402 |
Number of pages | 16 |
Journal | Epigenomics |
Volume | 9 |
Issue number | 11 |
DOIs | |
State | Published - Nov 2017 |
Keywords
- DNA methylation
- MRE-seq
- MeDIP-seq
- genome-wide
- metabolism
ASJC Scopus subject areas
- Genetics
- Cancer Research