Porcine intestinal lymphoid tissues synthesize estradiol

Chan Jin Park, Heehyen Kim, Jooyoung Jin, Radwa Barakat, Po Ching Lin, Jeong Moon Choi, Che Myong Jay Ko

Research output: Contribution to journalArticle

Abstract

Estradiol (17β-estradiol) is synthesized primarily in the gonads of both sexes and regulates the development and function of reproductive organs. Recently, we reported that intestinal lymphocyte homeostasis is regulated by estradiol synthesized de novo in the endothelial cells of the high endothelial venules (HEVs) of mesenteric lymph nodes and Peyer's patches in mice. This observation prompted us to hypothesize that HEVs of intestinal lymphoid tissues are sites of estradiol synthesis across species. In this study, we examined whether estradiol is synthesized in the intestinal lymphoid tissues of adolescent piglets. Comparisons of estradiol levels in blood and tissue showed that estradiol concentrations in mesenteric lymph nodes and Peyer's patches were significantly higher than the level in serum. Reverse transcription polymerase chain reaction showed that porcine intestinal lymphoid tissues express mRNAs for steroidogenic enzymes (StAR, 17β-Hsd, 3β-Hsd, Cyp17a1, and Cyp19a1), and immunohistochemical results in ilial tissue showed expression of aromatase (CYP19) in Peyer's patch-localized endothelial cells of HEVs. When mesenteric lymph node and Peyer's patch tissues were cultured in vitro, they produced estradiol. Taken together, the results indicate that mesenteric lymph nodes and Peyer's patches are sites of estradiol synthesis in adolescent piglets.

Original languageEnglish (US)
Pages (from-to)477-482
Number of pages6
JournalJournal of Veterinary Science
Volume19
Issue number4
DOIs
StatePublished - Jan 1 2018

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Keywords

  • Aromatase
  • Estradiol
  • Intestines
  • Lymph nodes
  • Swine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Park, C. J., Kim, H., Jin, J., Barakat, R., Lin, P. C., Choi, J. M., & Ko, C. M. J. (2018). Porcine intestinal lymphoid tissues synthesize estradiol. Journal of Veterinary Science, 19(4), 477-482. https://doi.org/10.4142/jvs.2018.19.4.477