Abstract

There have been a number of modifications to in vitro fertilization (IVF) systems to allow efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 20 years, the overall blastocyst production rate remains low. This is mainly due to two physiological events: polyspermic oocyte penetration and low rate of male pronucleus formation. Both of these pathological conditions appear to be inherent problems in the porcine in vitro production system and many of the mechanisms involved are still unknown. Many scientists have investigated the medium and techniques used during the various stages of in vitro production, but improvements to the equipment used during IVF remained unchanged until recently. This chapter discusses the preparation of spermatozoa, co-incubation times, changes in media composition, and development of modified equipment to improve the conditions used during IVF of porcine oocytes.

Original languageEnglish (US)
Pages (from-to)5-18
Number of pages14
JournalAnnual Review of Biomedical Sciences
Volume4
StatePublished - Dec 1 2002

Fingerprint

Fertilization in Vitro
Swine
Equipment and Supplies
Oocytes
Embryonic Structures
Blastocyst
Spermatozoa
In Vitro Techniques
Chemical analysis

Keywords

  • Embryo
  • In vitro fertilization
  • Polyspermy
  • Porcine

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Porcine in vitro fertilization : Advances in medium, components and equipment. / Clark, S. G.; Wheeler, Matthew B.

In: Annual Review of Biomedical Sciences, Vol. 4, 01.12.2002, p. 5-18.

Research output: Contribution to journalReview article

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AB - There have been a number of modifications to in vitro fertilization (IVF) systems to allow efficient production of viable porcine embryos. Although in vitro production of pig embryos has been studied for over 20 years, the overall blastocyst production rate remains low. This is mainly due to two physiological events: polyspermic oocyte penetration and low rate of male pronucleus formation. Both of these pathological conditions appear to be inherent problems in the porcine in vitro production system and many of the mechanisms involved are still unknown. Many scientists have investigated the medium and techniques used during the various stages of in vitro production, but improvements to the equipment used during IVF remained unchanged until recently. This chapter discusses the preparation of spermatozoa, co-incubation times, changes in media composition, and development of modified equipment to improve the conditions used during IVF of porcine oocytes.

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