Polysulfobetaine films prepared by electrografting technique for reduction of biofouling on electroconductive surfaces

Marek Stach, Zuzana Kroneková, Peter Kasák, Jozef Kollár, Martin Pentrák, Matej Mičušík, Dušan Chorvát, Tim S. Nunney, Igor Lacík

Research output: Contribution to journalArticlepeer-review

Abstract

The sulfobetaine films were prepared on stainless steel and golden surfaces. In the first step, the poly(2-(dimethylamino)ethyl methacrylate) film was created by employing the electrografting polymerization technique. In the second step, this film was modified to polysulfobetaine, i.e. the polymer film bearing the zwitterionic groups. The presence of the electrografted film and its modification were determined by contact angle measurements, infrared spectroscopy in reflectance mode and X-ray photoelectron spectroscopy. The prepared films were homogeneous with the thickness from about 5 to 26 nm as determined by X-ray photoelectron spectroscopy. The atomic force microscopy measurements showed the increase of surface roughness upon the surface coating. In vitro tests using adherent RAT-2 fibroblast cells and fluorescently labelled bovine serum albumin proteins showed that prepared polysulfobetaine films can be used in applications requiring the resistance against cell attachment and biofouling.

Original languageEnglish (US)
Pages (from-to)10795-10801
Number of pages7
JournalApplied Surface Science
Volume257
Issue number24
DOIs
StatePublished - Oct 1 2011
Externally publishedYes

Keywords

  • 2-(Dimethylamino)ethyl methacrylate
  • ATR-FTIR
  • Biofouling
  • CLSM
  • Contact angle
  • Electrografting
  • Sulfobetaine
  • XPS
  • Zwitterions

ASJC Scopus subject areas

  • General Chemistry
  • Condensed Matter Physics
  • General Physics and Astronomy
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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