Polyphosphate modulates blood coagulation and fibrinolysis

Stephanie A. Smith, Nicola J. Mutch, Deepak Baskar, Peter Rohloff, Roberto Docampo, James H. Morrissey

Research output: Contribution to journalArticlepeer-review

Abstract

Inorganic polyphosphate is an abundant component of acidocalcisomes of bacteria and unicellular eukaryotes. Human platelet dense granules strongly resemble acidocalcisomes, and we recently showed that they contain substantial amounts of polyphosphate, which is secreted upon platelet activation. We now report that polyphosphate is a potent hemostatic regulator, accelerating blood clotting by activating the contact pathway and promoting the activation of factor V, which in turn results in abrogation of the function of the natural anticoagulant protein, tissue factor pathway inhibitor. Polyphosphate was also found to delay clot lysis by enhancing a natural antifibrinolytic agent, thrombin-activatable fibrinolysis inhibitor. Polyphosphate is unstable in blood or plasma, owing to the presence of phosphatases. We propose that polyphosphate released from platelets or microorganisms initially promotes clot formation and stability; subsequent degradation of polyphosphate by blood phosphatases fosters inhibition of clotting and activation of fibrinolysis during wound healing.

Original languageEnglish (US)
Pages (from-to)903-908
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number4
DOIs
StatePublished - Jan 24 2006

Keywords

  • Acidocalcisomes
  • Dense granules
  • Factor V
  • Platelets
  • Tissue factor pathway inhibitor

ASJC Scopus subject areas

  • General

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