Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie

Shiquan Wang, Noelle E. Cockett, Janice M. Miller, Tracy L. Shay, Alma Maciulis, Diane L. Sutton, Warren C. Foote, Gilbert R. Holyoak, Ronald C. Evans, Thomas D. Bunch, Jonathan E. Beever, Jay W. Call, William D. Taylor, Michael R. Marshall

Research output: Contribution to journalArticle

Abstract

The genetic sequence of the ovine prion protein (PrP) gene between codons 102 and 175 with emphasis on ovine PrP gene codons 136 and 171 was determined, and the polymorphic distribution of the ovine PrP gene in the scrapie-exposed Suffolk embryo donors and offspring from these donors that were transferred to scrapie-free recipient ewes was investigated in this study. The most common genotype was AA136QQ171 (70% and 63% in the donor and offspring flocks, respectively), which is considered a high risk genotype in US Suffolk sheep, Although embryos were collected from scrapie- positive donors and many embryos had the high risk genotype, no scrapie occurred in the resulting offspring. Based upon the results of this study, we conclude that vertical transmission of scrapie can be circumvented using embryo transfer procedures even when the offspring have the high risk genotype.

Original languageEnglish (US)
Pages (from-to)1865-1875
Number of pages11
JournalTheriogenology
Volume57
Issue number7
DOIs
StatePublished - May 27 2002

Keywords

  • DNA sequence
  • Embryo transfer
  • Ovine prion protein gene (ovine PrP gene)
  • Scrapie
  • Suffolk

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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    Wang, S., Cockett, N. E., Miller, J. M., Shay, T. L., Maciulis, A., Sutton, D. L., Foote, W. C., Holyoak, G. R., Evans, R. C., Bunch, T. D., Beever, J. E., Call, J. W., Taylor, W. D., & Marshall, M. R. (2002). Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie. Theriogenology, 57(7), 1865-1875. https://doi.org/10.1016/S0093-691X(02)00673-8