Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie

Shiquan Wang; Noelle E. Cockett; Janice M. Miller; Tracy L. Shay; Alma Maciulis; Diane L. Sutton; Warren C. Foote; Gilbert R. Holyoak; Ronald C. Evans; Thomas D. Bunch; Jonathan E. Beever; Jay W. Call; William D. Taylor; Michael R. Marshall

doi:10.1016/S0093-691X(02)00673-8

Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie

Shiquan Wang, Noelle E. Cockett, Janice M. Miller, Tracy L. Shay, Alma Maciulis, Diane L. Sutton, Warren C. Foote, Gilbert R. Holyoak, Ronald C. Evans, Thomas D. Bunch, Jonathan E. Beever, Jay W. Call, William D. Taylor, Michael R. Marshall

Animal Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The genetic sequence of the ovine prion protein (PrP) gene between codons 102 and 175 with emphasis on ovine PrP gene codons 136 and 171 was determined, and the polymorphic distribution of the ovine PrP gene in the scrapie-exposed Suffolk embryo donors and offspring from these donors that were transferred to scrapie-free recipient ewes was investigated in this study. The most common genotype was AA₁₃₆QQ₁₇₁ (70% and 63% in the donor and offspring flocks, respectively), which is considered a high risk genotype in US Suffolk sheep, Although embryos were collected from scrapie- positive donors and many embryos had the high risk genotype, no scrapie occurred in the resulting offspring. Based upon the results of this study, we conclude that vertical transmission of scrapie can be circumvented using embryo transfer procedures even when the offspring have the high risk genotype.

Original language	English (US)
Pages (from-to)	1865-1875
Number of pages	11
Journal	Theriogenology
Volume	57
Issue number	7
DOIs	https://doi.org/10.1016/S0093-691X(02)00673-8
State	Published - 2002

Keywords

DNA sequence
Embryo transfer
Ovine prion protein gene (ovine PrP gene)
Scrapie
Suffolk

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

Online availability

10.1016/S0093-691X(02)00673-8

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Wang, S., Cockett, N. E., Miller, J. M., Shay, T. L., Maciulis, A., Sutton, D. L., Foote, W. C., Holyoak, G. R., Evans, R. C., Bunch, T. D., Beever, J. E., Call, J. W., Taylor, W. D., & Marshall, M. R. (2002). Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie. Theriogenology, 57(7), 1865-1875. https://doi.org/10.1016/S0093-691X(02)00673-8

Wang, S, Cockett, NE, Miller, JM, Shay, TL, Maciulis, A, Sutton, DL, Foote, WC, Holyoak, GR, Evans, RC, Bunch, TD, Beever, JE, Call, JW, Taylor, WD & Marshall, MR 2002, 'Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie', Theriogenology, vol. 57, no. 7, pp. 1865-1875. https://doi.org/10.1016/S0093-691X(02)00673-8

@article{a927d94bc83f4aa7802dcc290a1e8cbc,

title = "Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie",

abstract = "The genetic sequence of the ovine prion protein (PrP) gene between codons 102 and 175 with emphasis on ovine PrP gene codons 136 and 171 was determined, and the polymorphic distribution of the ovine PrP gene in the scrapie-exposed Suffolk embryo donors and offspring from these donors that were transferred to scrapie-free recipient ewes was investigated in this study. The most common genotype was AA136QQ171 (70% and 63% in the donor and offspring flocks, respectively), which is considered a high risk genotype in US Suffolk sheep, Although embryos were collected from scrapie- positive donors and many embryos had the high risk genotype, no scrapie occurred in the resulting offspring. Based upon the results of this study, we conclude that vertical transmission of scrapie can be circumvented using embryo transfer procedures even when the offspring have the high risk genotype.",

keywords = "DNA sequence, Embryo transfer, Ovine prion protein gene (ovine PrP gene), Scrapie, Suffolk",

author = "Shiquan Wang and Cockett, {Noelle E.} and Miller, {Janice M.} and Shay, {Tracy L.} and Alma Maciulis and Sutton, {Diane L.} and Foote, {Warren C.} and Holyoak, {Gilbert R.} and Evans, {Ronald C.} and Bunch, {Thomas D.} and Beever, {Jonathan E.} and Call, {Jay W.} and Taylor, {William D.} and Marshall, {Michael R.}",

note = "Funding Information: This research was supported by the USDA, APHIS, VS (Grant no. 94-9-1-49-0233-C), Utah Department of Agriculture (Grant no. 90-2720), and by the Utah Agricultural Experiment Station, Utah State University, Logan, UT 84322-4810. This manuscript was approved as Utah Agricultural Experiment Station journal paper number 7375. We thank Lyle Henroid and Dave Forrester, Department of Animal, Dairy and Veterinary Sciences, Utah State University, and personnel at the Scrapie Field Trial Station, USDA, APHIS, Mission, TX, for their help with sheep management and sample collection. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2002",

doi = "10.1016/S0093-691X(02)00673-8",

language = "English (US)",

volume = "57",

pages = "1865--1875",

journal = "Theriogenology",

issn = "0093-691X",

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T1 - Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie

AU - Wang, Shiquan

AU - Cockett, Noelle E.

AU - Miller, Janice M.

AU - Shay, Tracy L.

AU - Maciulis, Alma

AU - Sutton, Diane L.

AU - Foote, Warren C.

AU - Holyoak, Gilbert R.

AU - Evans, Ronald C.

AU - Bunch, Thomas D.

AU - Beever, Jonathan E.

AU - Call, Jay W.

AU - Taylor, William D.

AU - Marshall, Michael R.

N1 - Funding Information: This research was supported by the USDA, APHIS, VS (Grant no. 94-9-1-49-0233-C), Utah Department of Agriculture (Grant no. 90-2720), and by the Utah Agricultural Experiment Station, Utah State University, Logan, UT 84322-4810. This manuscript was approved as Utah Agricultural Experiment Station journal paper number 7375. We thank Lyle Henroid and Dave Forrester, Department of Animal, Dairy and Veterinary Sciences, Utah State University, and personnel at the Scrapie Field Trial Station, USDA, APHIS, Mission, TX, for their help with sheep management and sample collection. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2002

Y1 - 2002

N2 - The genetic sequence of the ovine prion protein (PrP) gene between codons 102 and 175 with emphasis on ovine PrP gene codons 136 and 171 was determined, and the polymorphic distribution of the ovine PrP gene in the scrapie-exposed Suffolk embryo donors and offspring from these donors that were transferred to scrapie-free recipient ewes was investigated in this study. The most common genotype was AA136QQ171 (70% and 63% in the donor and offspring flocks, respectively), which is considered a high risk genotype in US Suffolk sheep, Although embryos were collected from scrapie- positive donors and many embryos had the high risk genotype, no scrapie occurred in the resulting offspring. Based upon the results of this study, we conclude that vertical transmission of scrapie can be circumvented using embryo transfer procedures even when the offspring have the high risk genotype.

AB - The genetic sequence of the ovine prion protein (PrP) gene between codons 102 and 175 with emphasis on ovine PrP gene codons 136 and 171 was determined, and the polymorphic distribution of the ovine PrP gene in the scrapie-exposed Suffolk embryo donors and offspring from these donors that were transferred to scrapie-free recipient ewes was investigated in this study. The most common genotype was AA136QQ171 (70% and 63% in the donor and offspring flocks, respectively), which is considered a high risk genotype in US Suffolk sheep, Although embryos were collected from scrapie- positive donors and many embryos had the high risk genotype, no scrapie occurred in the resulting offspring. Based upon the results of this study, we conclude that vertical transmission of scrapie can be circumvented using embryo transfer procedures even when the offspring have the high risk genotype.

KW - DNA sequence

KW - Embryo transfer

KW - Ovine prion protein gene (ovine PrP gene)

KW - Scrapie

KW - Suffolk

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AN - SCOPUS:0035983715

SN - 0093-691X

VL - 57

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JO - Theriogenology

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IS - 7

ER -

Polymorphic distribution of the ovine prion protein (PrP) gene in scrapie-infected sheep flocks in which embryo transfer was used to circumvent the transmissions of scrapie

Abstract

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