Polymer cell culture substrates with combined nanotopographical patterns and micropatterned chemical domains

Joseph L. Charest, Marcus T. Eliason, Andŕs J. García, William P. King, A. Alec Talin, Blake A. Simmons

Research output: Contribution to journalArticlepeer-review

Abstract

A combination of nanoimprint lithography and microcontact printing was used to create cell substrates with well-defined nanotopographic patterns of grooves overlaid with independently controlled micropatterned chemical domains. Qualitative analysis of osteoblast-like cells cultured on the substrates showed alignment of cells and cell features to the nanotopographic grooves when surface chemistry was either uniform or a pattern of dots. When surface chemistry on the substrate was a pattern of lanes, cells aligned to the lanes. On all substrates, small cellular extensions, or filopodia, displayed no particular alignment to either nanotopographic grooves or chemical patterns. Large cell extensions were observed only parallel to either nanotopographic grooves or chemical lanes. The techniques used provide an easily scaleable approach to creating cell substrates that will aid in studying the relative impact and interplay of chemical patterns and mechanical topography on cellular responses.

Original languageEnglish (US)
Pages (from-to)3011-3014
Number of pages4
JournalJournal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures
Volume23
Issue number6
DOIs
StatePublished - Nov 2005
Externally publishedYes

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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