Poly(dimethylsiloxane) (PDMS) and silicon hybrid biochip for bacterial culture

Woo Jin Chang, Demir Akin, Miroslav Sedlak, Michael R. Ladisch, Rashid Bashir

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, a novel PDMS/silicon hybrid microfluidic biochip was fabricated and tested for the long-term batch culture of bacterial cells. The PDMS (poly(dimethylsiloxane)) cover with 3-dimensional micro-channels for flow was fabricated using Teflon tubing and hole-punch techniques, without photolithographic methods. The PDMS/silicon hybrid biochip was prepared by bonding of PDMS cover and a silicon chip that had electrodes and micro-fluidic channels defined. The absorption of liquid into PDMS cover was characterized and conditions to prevent drying of nutrient media within the micro-chamber were shown. The absorption of liquid from micro-chambers into the PDMS cover was reduced up to 2.5 times by changing the mixing ratio of PDMS and curing agent from 10 : 1 to 2.5 : 1. In addition, pre-saturation of the PDMS cover with media prior to the incubation resulted in the preservation of liquid in the micro-chambers for up to 22 hours. Optimization of the mixing ratio and pre-saturation of the PDMS cover reduced the drying time 10 times when compared to the unsaturated PDMS cover composed of 10 : 1 ratio of PDMS and curing agent. Listeria innocua and a strain of Escherichia coli, expressing green fluorescent protein (GFP), were successfully cultured in batch mode within the PDMS/silicon hybrid biochip.

Original languageEnglish (US)
Pages (from-to)281-290
Number of pages10
JournalBiomedical microdevices
Volume5
Issue number4
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Keywords

  • Absorption in PDMS
  • Bacterial culture
  • Fabrication of 3-dimensional micro-channel
  • PDMS/silicon hybrid biochip

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Genetics
  • Neuroscience(all)

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