Infrared spectroscopic imaging has emerged as a powerful label-free diagnostic tool to study the molecular composition and organization in biological tissues and cells. We report infrared spectroscopic imaging using polarized light to study differential absorption of plane-polarized light by an oriented sample to detect valuable information, such as, birefringence and dichroism. For instance, the organization of collagen, specifically fiber orientation and alignment, is crucial in understanding the progression and metastasis of cancer. Recent advancements in the development of Quantum Cascade Lasers (QCL) sources have opened new avenues for high SNR measurements in the field of IR spectroscopy. In addition, QCL sources are intrinsically polarized and orientation information can be obtained at discrete frequencies with different polarization orientations, allowing much faster acquisition than a corresponding FT-IR approach. We demonstrate improved performance in terms of fast and comprehensive polarimetric image acquisition and analysis using custom-built QCL microscope and evaluate its impact on applications by analyzing the important spectral bands of surgical tissue sections.

Original languageEnglish (US)
Title of host publicationAdvanced Chemical Microscopy for Life Science and Translational Medicine
EditorsJi-Xin Cheng, Wei Min, Garth J. Simpson
ISBN (Electronic)9781510632677
StatePublished - 2020
EventAdvanced Chemical Microscopy for Life Science and Translational Medicine 2020 - San Francisco, United States
Duration: Feb 1 2020Feb 3 2020

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceAdvanced Chemical Microscopy for Life Science and Translational Medicine 2020
Country/TerritoryUnited States
CitySan Francisco


  • Collagen
  • Degree of Polarization
  • IR Spectroscopic Imaging
  • Infrared Microscopy
  • Orientation
  • Polarization Factor
  • Polarization Imaging
  • Quantum Cascade Lasers

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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