A rapid and portable analytical methodology has been developed for ascorbic acid (Vitamin C) quantification from aqueous samples using a spectrometric smartphone-based system for the first time. The method employs point-of-use approaches both for sample preparation and sample measurement, demonstrating the capability for mobile quality control of pharmaceutical and food products. Our approach utilizes an oxidation–reduction reaction between ascorbic acid and methylene blue, followed by a dispersive liquid–liquid microextraction (DLLME) to extract the aqueous-phase methylene blue into organic media. Then, a back-extraction procedure is employed to transfer the methylene blue to aqueous media, followed by analysis of the sample's absorption spectrum using the spectrometric smartphone-based system. The DLLME and back-extraction procedures are optimized by use of a two-step multivariate optimization strategy. Finally, vitamin C supplements and orange juice are used as real-world samples to assess the applicability of the smartphone-based method, which is successfully compared with the standard laboratory-based approach.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalFood chemistry
StatePublished - Jan 30 2019


  • Multivariate optimization
  • Orange juice
  • Point-of-use detection
  • Smartphone-based system
  • Vitamin C quantification
  • Vitamin C supplements

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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