Ploidy levels of plants regenerated from mixed ploidy maize callus cultures

Y. Wan, L. M. McMurphy, A. L. Rayburn, J. M. Widholm

Research output: Contribution to journalArticlepeer-review


Haploid and diploid anther-derived Zea mays callus lines were treated with the antimicrotubule herbicide pronamide to produce mixed ploidy callus as determined by flow cytometry. The ploidy levels of the plants regenerated from the callus were determined by counting the leaf epidermal guard cell chloroplast numbers. The proportion of diploid regenerated plants was somewhat lower than the proportion of diploid cells of the callus. The diploid plants regenerated somewhat faster than the haploids. The proportion of tetraploids regenerated from the pronamide treated diploid callus, which originated by spontaneous chromosome doubling, was much lower than the proportion of cells indicating that tetraploid cells survive or regenerate plants at a lower frequency than diploid cells.

Original languageEnglish (US)
Pages (from-to)87-89
Number of pages3
JournalIn Vitro - Plant
Issue number2
StatePublished - Apr 1992


  • Zea mays
  • anther culture
  • callus culture
  • chromosome doubling
  • plant regeneration
  • pronamide

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science


Dive into the research topics of 'Ploidy levels of plants regenerated from mixed ploidy maize callus cultures'. Together they form a unique fingerprint.

Cite this